RHBDD1 upregulates EGFR via the AP-1 pathway in colorectal cancer.

Abstract

Our previous study showed that RHBDD1 can activate the EGFR signaling pathway to promote colorectal cancer growth. In the present study, EGFR was decreased when RHBDD1 was knocked down or inactivated. Further analysis found that c-Jun and EGFR protein expression was decreased in RHBDD1 knockdown and inactivated cells. c-Jun overexpression in RHBDD1-inactivated cells rescued EGFR expression in a dose-dependent manner. RHBDD1 overexpression in RHBDD1-inactivated cells restored EGFR expression, but this effect was counteracted by c-Jun knockdown. Furthermore, EGFR and c-Jun were attenuated in the RHBDD1 knockdown and inactivated groups in animal tumor models. Tissue microarray assays demonstrated a correlation between RHBDD1 and EGFR in colorectal cancer patients. Therefore, our findings indicate that RHBDD1 stimulates EGFR expression by promoting the AP-1 pathway.

• Authors: Fei Miao, Mengmeng Zhang, Yuechao Zhao, Xiaolu Li, Rongyan Yao, Fan Wu, Rong Huang, Kai Li, Shiying Miao, Changwu Ma, Hongge Ju, Wei Song, Linfang Wang
• Journal: Oncotarget (Oncotarget) Vol. 8 Issue 15 Pg. 25251-25260 (Apr 11 2017) ISSN: 1949-2553 [Electronic] United States
• PMID: 28445956 (Publication Type: Journal Article)

Chemical References

• Transcription Factor AP-1
• EGFR protein, human
• ErbB Receptors
• RHBDD1 protein, human
• Serine Endopeptidases

Topics

• Aged
• Animals
• Colorectal Neoplasms (genetics, pathology)
• ErbB Receptors (metabolism)
• Female
• HCT116 Cells
• Heterografts
• Humans
• Male
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Middle Aged
• Serine Endopeptidases (genetics, metabolism)
• Signal Transduction
• Transcription Factor AP-1 (genetics, metabolism)
• Transfection
• Up-Regulation