Biological activity and LC-MS/MS profiling of extracts from the Australian medicinal plant Acacia ligulata (Fabaceae).

Abstract

Acacia ligulata A.Cunn. ex Benth. (Fabaceae: Mimosoideae) is a native Australian plant used traditionally by Australian Aboriginal groups. This study was undertaken to investigate the bioactivity of A. ligulata extracts and to evaluate their chemical composition. Potential antibacterial, cytotoxic and enzyme inhibitory effects relevant to traditional medicinal and food uses of the species were examined and LC-MS/MS was performed to investigate the chemical composition. Antibacterial activity was observed for bark and leaf extracts with an MIC for the bark extract of 62.5 μg/mL against Streptococcus pyogenes. Pod extracts showed cytotoxic effects against cancer cells, with the highest activity against melanoma SK-MEL28 cells with IC50 values between 40.8 and 80.6 μg/mL. Further, the leaf and pod extracts also inhibited α-amylase EC-3.2.1.1 and α-glucosidase EC-3.2.1.20 with IC50 values between 9.7-34.8 and 12.6-64.3 μg/mL, respectively. The LC-MS/MS profiling indicated that several different saponins were present in the active extracts.

• Authors: Diana Jæger, Bradley S Simpson, Chi P Ndi, Anna K Jäger, Christoph Crocoll, Birger Lindberg Møller, Philip Weinstein, Susan J Semple
• Journal: Natural product research (Nat Prod Res) Vol. 32 Issue 5 Pg. 576-581 (Mar 2018) ISSN: 1478-6427 [Electronic] England
• PMID: 28427277 (Publication Type: Journal Article)

Chemical References

• Anti-Bacterial Agents
• Glycoside Hydrolase Inhibitors
• Plant Extracts
• Saponins
• alpha-Amylases
• alpha-Glucosidases

Topics

• Acacia (chemistry)
• Anti-Bacterial Agents (chemistry, pharmacology)
• Australia
• Cell Line, Tumor
• Chromatography, Liquid
• Drug Evaluation, Preclinical (methods)
• Glycoside Hydrolase Inhibitors (chemistry, pharmacology)
• Humans
• Inhibitory Concentration 50
• Microbial Sensitivity Tests
• Plant Bark (chemistry)
• Plant Extracts (analysis, chemistry)
• Plant Leaves (drug effects)
• Plants, Medicinal (chemistry)
• Saponins (analysis)
• Streptococcus pyogenes (drug effects)
• Tandem Mass Spectrometry (methods)
• alpha-Amylases (antagonists & inhibitors)
• alpha-Glucosidases (metabolism)