Down-regulated expression of OPCML predicts an unfavorable prognosis and promotes disease progression in human gastric cancer.

Abstract

BACKGROUND:

OPCML belongs to the IgLON family of Ig domain-containing GPI-anchored cell adhesion molecules and was recently found to be involved in carcinogenesis, while its role in gastric cancer remains unclear.

METHODS:

We assessed expression and biological behavior of OPCML in gastric cancer.

RESULTS:

OPCML expression was markedly reduced in tumor tissues and cancer cell lines. Decreased OPCML expression had a significant association with unfavorable tumor stage (p = 0.007) and grading (p < 0.001). Furthermore, the results revealed that OPCML was an independent prognostic factor for overall survival in gastric cancer (p = 0.002). In addition, ectopic expression of OPCML in cancer cells significantly inhibited cell viability (p < 0.01) and colony formation (p < 0.001), arrest cell cycle in G0/G1 phase and induced apoptosis, and suppressed tumor formation in nude mice. The alterations of phosphorylation status of AKT and its substrate GSK3β, up-regulation of pro-apoptotic regulators including caspase-3, caspase-9 and PARP, and up-regulation of cell cycle regulator p27, were implicated in the biological activity of OPCML in cancer cells.

CONCLUSION:

Down-regulated OPCML expression might serve as an independent predictor for unfavorable prognosis of patients, and the biological behavior supports its role as a tumor suppressor in gastric cancer.

• Authors: Xiangbin Xing, Weibin Cai, Sanmei Ma, Yongfei Wang, Huijuan Shi, Ming Li, Jinxia Jiao, Yang Yang, Longshan Liu, Xiangliang Zhang, Minhu Chen
• Journal: BMC cancer (BMC Cancer) Vol. 17 Issue 1 Pg. 268 (Apr 14 2017) ISSN: 1471-2407 [Electronic] England
• PMID: 28407749 (Publication Type: Journal Article)

Chemical References

• Cell Adhesion Molecules
• GPI-Linked Proteins
• OPCML protein, human
• GSK3B protein, human
• Glycogen Synthase Kinase 3 beta
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Cell Adhesion Molecules (genetics, metabolism)
• Cell Line, Tumor
• Cell Survival
• Disease Progression
• Down-Regulation
• Female
• GPI-Linked Proteins (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Glycogen Synthase Kinase 3 beta (metabolism)
• Humans
• Male
• Mice
• Neoplasm Transplantation
• Prognosis
• Proto-Oncogene Proteins c-akt (metabolism)
• Signal Transduction
• Stomach Neoplasms (genetics, metabolism, pathology)