Maspin suppresses growth, proliferation and invasion in cutaneous squamous cell carcinoma cells.

Abstract

Cutaneous squamous cell carcinoma (cSCC) is a common malignant tumor. Mammary serine protease inhibitor (Maspin), a member of serpin family, has been reported as a tumor suppressor in various carcinomas. In this study, we detected the expression level of Maspin in cSCC tissues by real-time PCR and western blotting, and found that Maspin was downregulated in the cSCC tissues compared with the adjacent normal tissues. Moreover, Maspin was stably overexpressed in A431 cells, and CCK-8 assay, colony formation assay, Transwell assay, Hoechst staining and western blotting were carried out to detect the growth, proliferation, invasion, cell cycle and apoptosis of A431 cells. The results revealed that overexpression of Maspin inhibited growth, proliferation, invasion and cell cycle G1/S/G2 transition and enhanced apoptosis of A431 cells. The pro-apoptotic protein cleaved caspase-3, poly(ADP-ribose) polymerase (PARP) and Bax increased, and the anti-apoptotic protein Bcl-2 decreased after Maspin overexpression. Therefore, we demonstrated that Maspin suppressed growth, proliferation and invasion by delaying cell cycle transition and promoting apoptosis in cSCC cells, which may provide new insights for the clinical diagnosis and therapy of cSCC.

• Authors: Hong Zhu, Qing Mao, Weiwei Liu, Zhenhai Yang, Xiaoqing Jian, Le Qu, Chundi He
• Journal: Oncology reports (Oncol Rep) Vol. 37 Issue 5 Pg. 2875-2882 (May 2017) ISSN: 1791-2431 [Electronic] Greece
• PMID: 28405681 (Publication Type: Journal Article)

Chemical References

• SERPIN-B5
• Serpins

Topics

• Carcinoma, Squamous Cell (genetics, metabolism)
• Cell Cycle
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation
• Gene Expression Regulation, Neoplastic
• Humans
• Neoplasm Invasiveness
• Serpins (genetics, metabolism)
• Skin Neoplasms (genetics, metabolism)