Quantitative proteomic studies in resistance mechanisms of Eimeria tenella against polyether ionophores.

Abstract

Polyether ionophores are widely used to treat and control coccidiosis in chickens. Widespread use of anticoccidials resulted in worldwide resistance. Mechanisms of resistance development and expansion are complex and poorly understood. Relative proteomic quantification using LC-MS/MS was used to compare sensitive reference strains (Ref-1, Ref-2) with putatively resistant and moderately sensitive field strains (FS-R, FS-mS) of Eimeria tenella after isotopic labelling with tandem mass tags (TMT). Ninety-seven proteins were identified, and 25 of them were regulated. Actin was significantly upregulated in resistant strains in comparison with their sensitive counterparts. On the other hand, microneme protein (MIC4) was downregulated in resistant strains. Optimization of labelling E. tenella sporozoites by TMT might identify further proteins that play a role in the obvious complex mechanism leading to resistance against Monensin.

• Authors: Ahmed Thabet, Walther Honscha, Arwid Daugschies, Berit Bangoura
• Journal: Parasitology research (Parasitol Res) Vol. 116 Issue 5 Pg. 1553-1559 (May 2017) ISSN: 1432-1955 [Electronic] Germany
• PMID: 28352944 (Publication Type: Journal Article)

Chemical References

• Actins
• Coccidiostats
• Ionophores
• Protozoan Proteins
• Monensin

Topics

• Actins (biosynthesis)
• Animals
• Chickens (parasitology)
• Coccidiosis (drug therapy, veterinary)
• Coccidiostats (pharmacology)
• Drug Resistance
• Eimeria tenella (drug effects)
• Ionophores (pharmacology)
• Monensin (pharmacology)
• Poultry Diseases (drug therapy)
• Proteomics
• Protozoan Proteins (biosynthesis)
• Sporozoites (drug effects)
• Tandem Mass Spectrometry