lncRNA MEG3 had anti-cancer effects to suppress pancreatic cancer activity.

Abstract

AIM:

The aim of this study was to explain the mechanism of lncRNA MEG 3 in pancreatic cancer.

METHODS:

We were collecting 30 pancreatic cancer patients, taking the sample from these patients. We measured the PI3K protein expressions from 30 patients by IHC and WB methods and MEG 3 expression by RT-PCR, and analyzed the relationship between PI3K protein expression and pancreatic cancer patients' clinical pathology and the correlation between lncRNA MEG 3 and PI3K. In the cell experiment, PANC-1 cells were divided into three groups: NC, BL and lncRNA groups, after treatment,we measured cell proliferation rate of 3 groups by MTT methods, evaluated cell apoptosis and cell cycle using flow cytometry, tested the invasion cells and migrate rate of 3 groups by transwell and wound healing assays.

RESULTS:

Compared with carcinoma adjacent tissue, The PI3K protein expression of pancreatic cancer tissue were significantly up-regulation (P>0.05). MEG 3 gene expression was negatively correlated with PI3K expression. The MEG 3 was negatively correlated with tumor size, Metastasis and Vascular invasion in pancreatic cancer (P<0.05, respectively). In the cell experiment, The cell proliferation and apoptosis rates of lncRNA group were significantly difference compared with NC group (P<0.05, respectively), and the G1 phase rate of lncRNA group was higher than NC group (P<0.05). The invasion cells and wound healing rate were significantly reduced in lncRNA group than those in NC group (P<0.05, respectively).

CONCLUSION:

MEG 3 over-expressing had anti-cancer effects to suppress pancreatic cancer activity by regulation PI3K/AKT/Bcl-2/Bax/Cyclin D1/P53 and PI3K/AKT/MMP-2/MMP-9 signaling pathways.

• Authors: Lei Gu, Jiaqiang Zhang, Minmin Shi, Qian Zhan, Baiyong Shen, Chenghong Peng
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 89 Pg. 1269-1276 (May 2017) ISSN: 1950-6007 [Electronic] France
• PMID: 28320094 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Chemical References

• Apoptosis Regulatory Proteins
• MEG3 non-coding RNA, human
• RNA, Long Noncoding
• Phosphatidylinositol 3-Kinases
• Proto-Oncogene Proteins c-akt
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Apoptosis (genetics)
• Apoptosis Regulatory Proteins (genetics)
• Cell Line, Tumor
• Cell Movement (genetics)
• Cell Proliferation (genetics)
• G1 Phase (genetics)
• Gene Expression (genetics)
• Humans
• Matrix Metalloproteinase 2 (genetics)
• Matrix Metalloproteinase 9 (genetics)
• Neoplasm Invasiveness (genetics)
• Pancreatic Neoplasms (genetics, pathology)
• Phosphatidylinositol 3-Kinases (genetics)
• Proto-Oncogene Proteins c-akt (genetics)
• RNA, Long Noncoding (genetics)
• Signal Transduction (genetics)