Down-regulation of lncRNA CASC2 promotes cell proliferation and metastasis of bladder cancer by activation of the Wnt/β-catenin signaling pathway.

Abstract

Long noncoding RNAs cancer susceptibility candidate 2 (CASC2) have been demonstrated as playing crucial regulatory roles in a few of cancers. However, the biological function of lncRNA CASC2 in bladder cancer are still unclear. In this study, we found that lncRNA CASC2 was significantly down-regulated in bladder cancer tissues and cell lines by quantitative real time-PCR and associated with advanced TNM stage (III/IV). Moreover, overexpression of lncRNA CASC2 remarkably reduced the cell growth, migration and invasion, as well as promoted early apoptosis of bladder cancer cell in vitro. Furthermore, we illustrated that lncRNA CASC2 inhibited Wnt/β-catenin signal pathway activity by decrasing the β-catenin expression and reversing the downstream target gene expression of Wnt signaling pathway. Taken together, lncRNA CASC2 plays an pivotal role in bladder tumorigenesis and progression and may act as a potential biomarker for the treatment of bladder cancer.

• Authors: Zhijun Pei, Xian Du, Yafeng Song, Lin Fan, Fuyan Li, Yan Gao, Ruimin Wu, Yijia Chen, Wei Li, Hong Zhou, Yi Yang, Jing Zeng
• Journal: Oncotarget (Oncotarget) Vol. 8 Issue 11 Pg. 18145-18153 (Mar 14 2017) ISSN: 1949-2553 [Electronic] United States
• PMID: 28199978 (Publication Type: Journal Article)

Chemical References

• RNA, Long Noncoding
• Tumor Suppressor Proteins
• long non-coding RNA CASC2, human

Topics

• Blotting, Western
• Cell Line, Tumor
• Cell Proliferation
• Down-Regulation
• Flow Cytometry
• Gene Expression Regulation, Neoplastic (physiology)
• Humans
• Neoplasm Invasiveness (genetics)
• RNA, Long Noncoding (genetics)
• Real-Time Polymerase Chain Reaction
• Tumor Suppressor Proteins (biosynthesis)
• Urinary Bladder Neoplasms (genetics, metabolism, pathology)
• Wnt Signaling Pathway (physiology)