Abstract |
Pathology are still progressive and cumulative in the remission course of relapsing-remitting MS (RRMS), thus drug treatment during the remission period may play a great role for the regeneration of the myelin sheath. C57BL/6 mice were fed with cuprizone (CPZ, 0.2% w/w) for 5 weeks to induce acute demyelination and oligodendrocytes degeneration, after which CPZ was withdrawn to allow recovery. Icariin (ICA, 6.25, 12.5 and 25mg/kg/day), vehicle (0.5% sodium carboxymethyl cellulose solution) or water was administrated orally to mice for 1 week after CPZ withdrawal. Luxol-fast blue (LFB), immunohistochemical or immunofluorescence staining was used to detect morphological and biological changes in the brains. CPZ administration for 5 weeks resulted in completely demyelination and remyelination occurred when CPZ was withdrawn. ICA treatment during the recovery period for 1 week significantly improved myelin restoration, enhanced NF200-positive axons repair, increased the number of APC+/Olig2+ mature oligodendrocytes and prevented neuron-derived neurotrophic factor such as nerve growth factor ( NGF) loss. Our results demonstrated that ICA treatment during the recovery period promotes remyelination and axon rewrapped, at least, in part, by promoting oligodendrogenesis and neurotrophic factor production.
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Authors | Yifan Zhang, Linlin Yin, Na Zheng, Li Zhang, Jianghong Liu, Weixiong Liang, Qi Wang |
Journal | Brain research bulletin
(Brain Res Bull)
Vol. 130
Pg. 180-187
(04 2017)
ISSN: 1873-2747 [Electronic] United States |
PMID | 28161197
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2017 Elsevier Inc. All rights reserved. |
Chemical References |
- Flavonoids
- Cuprizone
- Nerve Growth Factor
- icariin
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Topics |
- Animals
- Axons
(drug effects, pathology)
- Brain
(drug effects, pathology)
- Cuprizone
(administration & dosage)
- Demyelinating Diseases
(chemically induced, pathology, prevention & control)
- Female
- Flavonoids
(administration & dosage)
- Frontal Lobe
(drug effects, metabolism)
- Mice, Inbred C57BL
- Nerve Growth Factor
(metabolism)
- Neurons
(drug effects, metabolism)
- Oligodendroglia
(drug effects, pathology)
- Remyelination
(drug effects)
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