Akt/mTOR mediated induction of bystander effect signaling in a nucleus independent manner in irradiated human lung adenocarcinoma epithelial cells.

Abstract

Cytoplasm is an important target for the radiation-induced bystander effect (RIBE). In the present work, the critical role of protein kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway in the generation of RIBE signaling after X-ray irradiation and the rapid phosphorylation of Akt and mTOR was observed in the cytoplasm of irradiated human lung adenocarcinoma epithelial (A549) cells. Targeting A549 cytoplasts with individual protons from a microbeam showed that RIBE signal(s) mediated by the Akt/mTOR pathway were generated even in the absence of a cell nucleus. These results provide a new insight into the mechanisms driving the cytoplasmic response to irradiation and their impact on the production of RIBE signal(s).

• Authors: Lu Li, Lu Wang, Kevin M Prise, K N Yu, Guodong Chen, Lianyun Chen, Yide Mei, Wei Han
• Journal: Oncotarget (Oncotarget) Vol. 8 Issue 11 Pg. 18010-18020 (Mar 14 2017) ISSN: 1949-2553 [Electronic] United States
• PMID: 28152510 (Publication Type: Journal Article)

Chemical References

• MTOR protein, human
• Proto-Oncogene Proteins c-akt
• TOR Serine-Threonine Kinases

Topics

• A549 Cells
• Adenocarcinoma (metabolism, pathology)
• Adenocarcinoma of Lung
• Blotting, Western
• Bystander Effect (physiology)
• Cell Nucleus
• Coculture Techniques
• Cytoplasm (metabolism, radiation effects)
• Fluorescent Antibody Technique
• Humans
• Lung Neoplasms (metabolism, pathology)
• Proto-Oncogene Proteins c-akt (metabolism)
• TOR Serine-Threonine Kinases (metabolism)