Silencing of IRF3 alleviates chronic neuropathic pain following chronic constriction injury.

Abstract

Interferon regulatory factor 3 (IRF3) is a member of IRF family which plays an important role in neuronal survival and neuroprotection. However, the role of IRF3 in neuropathic pain remains unclear. Thus, in this study, we investigated the effect of IRF3 on neuropathic pain in a rat chronic constriction injury (CCI) model. Our results showed that IRF3 was up-regulated in the dorsal root ganglion (DRG) in CCI rats. Intrathecal short-hairpin RNA (shRNA)-IRF3 attenuated mechanical allodynia and thermal hyperalgesia in CCI rats, as well as inhibited the production of TNF-α and IL-1β in the DRG of CCI rats. Furthermore, we revealed that sh-IRF3 greatly suppressed the expression of p-NF-κB p65 and IκBα degradation in the DRG of CCI rats. In conclusion, our data suggest that knockdown of IRF3 may alleviate neuropathic pain by inhibiting the activation of NF-κB signaling pathway. Therefore, IRF3 may provide an important target for the treatment of neuropathic pain.

• Authors: Rui Li, Li-Guo Wang, Qi Wang, Zhi-Hua Li, Ya-Li Ma, Qing-Duo Guo
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 88 Pg. 403-408 (Apr 2017) ISSN: 1950-6007 [Electronic] France
• PMID: 28122305 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Chemical References

• Cytokines
• Inflammation Mediators
• Interferon Regulatory Factor-3
• NF-kappa B

Topics

• Animals
• Chronic Pain (complications, metabolism, pathology)
• Constriction
• Cytokines (metabolism)
• Down-Regulation
• Ganglia, Spinal (pathology)
• Gene Silencing
• Hyperalgesia (complications)
• Inflammation Mediators (metabolism)
• Interferon Regulatory Factor-3 (metabolism)
• Male
• NF-kappa B (metabolism)
• Neuralgia (complications, metabolism, pathology)
• Rats, Sprague-Dawley
• Up-Regulation