Abstract |
Metformin, as an AMP-activated protein kinase (AMPK) activator, can activate autophagy. A study showed that metformin decreased the risk of hepatocellular carcinoma (HCC) in diabetic patients. However, the detailed mechanism in the metformin-mediated anticancer effect remains an open question. Transcription factor CCAAT/enhancer-binding protein delta (CEBPD) has been suggested to serve as a tumor suppressor and is responsive to multiple anticancer drugs in HCC. In this study, we found that CEBPD and autophagy are involved in metformin-induced cell apoptosis in Huh7 cells. The underlying mechanisms in this process included a reduction in Src-mediated CEBPD protein degradation and an increase in CEBPD-regulated LC3B and ATG3 gene transcription under metformin treatment. We also found that AMPK is involved in metformin-induced CEBPD expression. Combined treatment with metformin and rapamycin can enhance autophagic cell death through the AMPK-dependent and AMPK-independent pathway, respectively. Taken together, we provide a new insight and therapeutic approach by targeting autophagy in the treatment of HCC.
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Authors | Hsin-Hwa Tsai, Hong-Yue Lai, Yueh-Chiu Chen, Chien-Feng Li, Huei-Sheng Huang, Hsiao-Sheng Liu, Yau-Sheng Tsai, Ju-Ming Wang |
Journal | Oncotarget
(Oncotarget)
Vol. 8
Issue 8
Pg. 13832-13845
(Feb 21 2017)
ISSN: 1949-2553 [Electronic] United States |
PMID | 28099155
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents
- CEBPD protein, human
- Hypoglycemic Agents
- CCAAT-Enhancer-Binding Protein-delta
- Metformin
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Topics |
- Animals
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Autophagy
(drug effects)
- Blotting, Western
- CCAAT-Enhancer-Binding Protein-delta
(metabolism)
- Carcinoma, Hepatocellular
(metabolism)
- Cell Line, Tumor
- Chromatin Immunoprecipitation
- Flow Cytometry
- Gene Knockdown Techniques
- Heterografts
- Humans
- Hypoglycemic Agents
(pharmacology)
- Liver Neoplasms
(metabolism)
- Male
- Metformin
(pharmacology)
- Mice
- Mice, Inbred NOD
- Mice, SCID
- Microscopy, Fluorescence
- Polymerase Chain Reaction
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