Evidence for Feedback Regulation Following Cholesterol Lowering Therapy in a Prostate Cancer Xenograft Model.
Abstract | BACKGROUND: METHODS: Proliferation assays were conducted by MTS solution and assessed by Student's t-test. 90 male nude mice were placed on a high- cholesterol Western-diet for 7 days then injected subcutaneously with 1 × 105 LAPC-4 cells. Two weeks post-injection, mice were randomized to control, 11 mg/kg/day simvastatin, 30 mg/kg ezetimibe, or the combination and sacrificed 42 days post-randomization. We used a generalized linear model with the predictor variables of treatment, time, and treatment by time (i.e., interaction term) with tumor volume as the outcome variable. Total serum and tumor cholesterol were measured. Tumoral RNA was extracted and cDNA synthesized from 1 ug of total RNA for quantitative real-time PCR. RESULTS: CONCLUSIONS:
|
Authors | Elizabeth M Masko, Mahmoud A Alfaqih, Keith R Solomon, William T Barry, Christopher B Newgard, Michael J Muehlbauer, Nikolaos A Valilis, Tameika E Phillips, Susan H Poulton, Alexis R Freedland, Stephanie Sun, Shweta K Dambal, Sergio E Sanders, Everardo Macias, Michael R Freeman, Mark W Dewhirst, Salvatore V Pizzo, Stephen J Freedland |
Journal | The Prostate
(Prostate)
Vol. 77
Issue 5
Pg. 446-457
(04 2017)
ISSN: 1097-0045 [Electronic] United States |
PMID | 27900797
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, N.I.H., Extramural)
|
Copyright | © 2016 Wiley Periodicals, Inc. |
Chemical References |
- Anticholesteremic Agents
- Cholesterol
- Simvastatin
- Ezetimibe
|
Topics |
- Animals
- Anticholesteremic Agents
(administration & dosage)
- Cell Line, Tumor
- Cell Proliferation
(drug effects, physiology)
- Cholesterol
(blood)
- Drug Therapy, Combination
- Ezetimibe
(administration & dosage)
- Feedback, Physiological
(drug effects, physiology)
- Humans
- Male
- Mice
- Mice, Nude
- Prostatic Neoplasms
(blood, drug therapy)
- Simvastatin
(administration & dosage)
- Tumor Burden
(drug effects, physiology)
- Xenograft Model Antitumor Assays
(methods)
|
|
Join CureHunter, for free Research Interface BASIC access!
Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease.
Find out why thousands of doctors, pharma researchers and patient activists
around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!
|