Knockdown of long non-coding RNA CCAT2 suppressed proliferation and migration of glioma cells.

Abstract

Long non-coding RNA colon cancer-associated transcript 2 (CCAT2) is commonly investigated in a number of cancers. However, little is known of its expression and biological function in glioma biology. In the current study, we used quantitative real-time PCR (qRT-PCR) to determine the expression of CCAT2 in glioma tissues. We found that expression of CCAT2 was up-regulated in glioma tissues and significantly correlated with the advanced tumor stage (III/IV). Functional assays in vitro and in vivo demonstrated that knockdown of CCAT2 could inhibit proliferation, cell cycle progression and migration of glioma cells. Further analysis indicated the effect of CCAT2 knockdown on glioma cell phenotype through inhibiting Wnt/β-catenin signal pathway activity. Thus, our study provides evidence that CCAT2 may function as a potential biomarker for glioma.

• Authors: Hua Guo, Guowen Hu, Qing Yang, Pei Zhang, Wei Kuang, Xingen Zhu, Lei Wu
• Journal: Oncotarget (Oncotarget) Vol. 7 Issue 49 Pg. 81806-81814 (Dec 06 2016) ISSN: 1949-2553 [Electronic] United States
• PMID: 27833083 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• RNA, Long Noncoding
• long non-coding RNA CCAT2, human

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Animals
• Biomarkers, Tumor (genetics, metabolism)
• Brain Neoplasms (genetics, metabolism, pathology)
• Cell Cycle
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Child
• Down-Regulation
• Female
• Gene Expression Regulation, Neoplastic
• Gene Knockdown Techniques
• Glioma (genetics, metabolism, pathology)
• Humans
• Male
• Mice, Inbred BALB C
• Mice, Nude
• Middle Aged
• Neoplasm Invasiveness
• Neoplasm Staging
• RNA Interference
• RNA, Long Noncoding (genetics, metabolism)
• Real-Time Polymerase Chain Reaction
• Time Factors
• Transfection
• Wnt Signaling Pathway
• Young Adult