Mastoparan is a membranolytic anti-cancer peptide that works synergistically with gemcitabine in a mouse model of mammary carcinoma.

Abstract

Anti-cancer peptides (ACPs) are small cationic and hydrophobic peptides that are more toxic to cancer cells than normal cells. ACPs kill cancer cells by causing irreparable membrane damage and cell lysis, or by inducing apoptosis. Direct-acting ACPs do not bind to a unique receptor, but are rather attracted to several different molecules on the surface of cancer cells. Here we report that an amidated wasp venom peptide, Mastoparan, exhibited potent anti-cancer activities toward leukemia (IC50~8-9.2μM), myeloma (IC50~11μM), and breast cancer cells (IC50~20-24μM), including multidrug resistant and slow growing cancer cells. Importantly, the potency and mechanism of cancer cell killing was related to the amidation of the C-terminal carboxyl group. Mastoparan was less toxic to normal cells than it was to cancer cells (e.g., IC50 to PBMC=48μM). Mastoparan killed cancer cells by a lytic mechanism. Moreover, Mastoparan enhanced etoposide-induced cell death in vitro. Our data also suggest that Mastoparan and gemcitabine work synergistically in a mouse model of mammary carcinoma. Collectively, these data demonstrate that Mastoparan is a broad-spectrum, direct-acting ACP that warrants additional study as a new therapeutic agent for the treatment of various cancers.

• Authors: Ashley L Hilchie, Andrew J Sharon, Evan F Haney, David W Hoskin, Marcel B Bally, Octavio L Franco, Jennifer A Corcoran, Robert E W Hancock
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1858 Issue 12 Pg. 3195-3204 (12 2016) ISSN: 0006-3002 [Print] Netherlands
• PMID: 27693190 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents
• Intercellular Signaling Peptides and Proteins
• Peptides
• Wasp Venoms
• Deoxycytidine
• mastoparan
• Gemcitabine

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Cell Line, Tumor
• Cell Membrane (drug effects)
• Circular Dichroism
• Deoxycytidine (analogs & derivatives, pharmacology)
• Drug Synergism
• Female
• Humans
• Intercellular Signaling Peptides and Proteins
• Mammary Neoplasms, Experimental (drug therapy, pathology)
• Mice
• Mice, Inbred BALB C
• Peptides (pharmacology)
• Wasp Venoms (pharmacology)
• Gemcitabine