Abstract |
In the current study, we have characterized the global miRNA expression profile in mouse pancreatic acinar cells and during acute pancreatitis using next-generation RNA sequencing. We identified 324 known and six novel miRNAs that are expressed in mouse pancreatic acinar cells. In the basal state, miR-148a-3p, miR-375-3p, miR-217-5p, and miR-200a-3p were among the most abundantly expressed, whereas miR-24-5p and miR-421-3p were the least abundant. Treatment of acinar cells with caerulein (100 nM) and taurolithocholic acid 3-sulfate [ TLC-S (250 μM)] induced numerous changes in miRNA expression profile. In particular, we found significant overexpression of miR-21-3p in acini treated with caerulein and TLC-S. We further looked at the expression of miR-21-3p in caerulein, l-arginine, and caerulein + LPS-induced acute pancreatitis mouse models and found 12-, 21-, and 50-fold increased expression in the pancreas, respectively. In summary, this is the first comprehensive analysis of global miRNA expression profile of mouse pancreatic acinar cells in normal and disease conditions. Our analysis shows that miR-21-3p expression level correlates with the severity of the disease.
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Authors | Ajay Kumar Dixit, Anne E Sarver, Zuobiao Yuan, John George, Usman Barlass, Hassam Cheema, Archana Sareen, Sulagna Banerjee, Vikas Dudeja, Rajinder Dawra, Subbaya Subramanian, Ashok K Saluja |
Journal | American journal of physiology. Gastrointestinal and liver physiology
(Am J Physiol Gastrointest Liver Physiol)
Vol. 311
Issue 5
Pg. G974-G980
(11 01 2016)
ISSN: 1522-1547 [Electronic] United States |
PMID | 27686613
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2016 the American Physiological Society. |
Chemical References |
- MicroRNAs
- taurolithocholic acid 3-sulfate
- Taurolithocholic Acid
- Ceruletide
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Topics |
- Acinar Cells
(drug effects, metabolism)
- Animals
- Ceruletide
(pharmacology)
- Gene Expression
(drug effects)
- Gene Expression Profiling
- High-Throughput Nucleotide Sequencing
- Mice
- MicroRNAs
(genetics, metabolism)
- Pancreatitis
(genetics, metabolism)
- Taurolithocholic Acid
(analogs & derivatives, pharmacology)
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