Inhibitory effects of dietary soyasaponin on 2,4-dinitrofluorobenzene-induced contact hypersensitivity in mice.

Abstract

Soyasaponins (SSs) abundant in soybean have anti-inflammatory activities; however, their therapeutic effects on allergic contact dermatitis (ACD) remain unknown. To assess the effects of SS-enriched diets on ACD, we used a mouse model of contact hypersensitivity (CHS). Mice were fed low-dose or high-dose SS-containing diets for 3 weeks prior to CHS induction with 2,4-dinitrofluorobenzene (DNFB). The low-dose SS diet attenuated DNFB-induced ear swelling and tissue oedema, and reduced the number of infiltrating Gr-1-positive myeloid cells. Low-dose, but not high-dose, SSs decreased chemokine (C-X-C motif) ligand 2 (CXCL2) and triggering receptor expressed on myeloid cells (TREM)-1 production in ear tissues, compared to a control. Taxonomic 16S rRNA analysis revealed significant alterations in faecal microbiota caused by CHS, which were reversed by low-dose SSs. The low-dose SS and non-CHS groups clustered together, while the high-dose SS group split between CHS and non-CHS clusters. Our results demonstrated that low-dose SSs alleviated CHS symptoms by attenuating inflammation and improving the intestinal microbiota composition, suggesting that dietary SSs may have beneficial effects on ACD.

• Authors: Takao Nagano, Mitsuru Katase, Kazunobu Tsumura, Mineki Saito, Tsukasa Matsuda
• Journal: Experimental dermatology (Exp Dermatol) Vol. 26 Issue 3 Pg. 249-254 (03 2017) ISSN: 1600-0625 [Electronic] Denmark
• PMID: 27618807 (Publication Type: Journal Article)
• Copyright: © 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Chemokine CXCL2
• Cxcl2 protein, mouse
• Saponins
• TREM1 protein, mouse
• Triggering Receptor Expressed on Myeloid Cells-1
• Dinitrofluorobenzene

Topics

• Animals
• Chemokine CXCL2 (metabolism)
• Dermatitis, Contact (drug therapy, pathology)
• Dietary Supplements
• Dinitrofluorobenzene
• Feces (microbiology)
• Female
• Mice
• Mice, Inbred BALB C
• Saponins (administration & dosage, therapeutic use)
• Glycine max
• Triggering Receptor Expressed on Myeloid Cells-1 (metabolism)