Arc discharge-mediated disassembly of viral particles in water.

Abstract

In this study, we investigated the inactivation effects on murine norovirus (MNV-1) with/without purification in water using a submerged plasma reactor of arc discharge (underwater arc), which produced a shockwave, UV light, reactive oxygen species and reactive nitrogen species. Underwater arc treatments of 3 and 6 Hz at 12 kV resulted in 2.6- and 4.2-log reductions in the virus titer of non-purified MNV-1 after 1 min of treatment, respectively. The reduction of purified MNV-1 was higher than that of non-purified MNV-1 after underwater arc treatment for all applied conditions (12 or 15 kV and 3 or 6 Hz). One of the viral capsid proteins (VP1) was not detectable after underwater arc treatment, when its integrity was assessed by western blot analysis. Transmission electron microscopy analysis also revealed that MNV-1 particles were completely dissembled by the treatment. This study demonstrates that underwater arc treatment, which was capable of disintegrating the MNV-1 virion structure and the viral capsid protein, can be an effective disinfection process for the inactivation of water-borne noroviruses.

• Authors: Eun-Jung Lee, Wooseong Lee, Minwoo Kim, Eun Ha Choi, Yun-Ji Kim
• Journal: Water research (Water Res) Vol. 102 Pg. 305-312 (10 01 2016) ISSN: 1879-2448 [Electronic] England
• PMID: 27379726 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• Water

Topics

• Animals
• Disinfection
• Mice
• Norovirus (isolation & purification)
• Virion
• Virus Inactivation
• Water