We describe a novel strategy to fabricate a well-defined
polydiacetylene (PDA) supramolecular pattern on
polyvinylidene fluoride (
PVDF) membrane utilizing
a DNA bio-photomask. By focusing on that, the absorption spectrum of
DNA molecules having a λmax at 260 nm overlaps with the wavelength at which the photopolymerization of the diacetylene monomer occurs,
DNA molecules are used to define specific patterns on
PVDF membranes coated with diacetylene
lipids by shielding the applied 254 nm UV light and consequently preventing the photopolymerization of the
lipids. As a result, the
DNA-covered regions retain the original white color on the membrane while the regions uncovered by
DNA molecules undergo the color change to blue through the photopolymerization by 254 nm UV irradiation. On the basis of the selective illumination through
a DNA photomask, we precisely manufacture specific patterns using a microarray
spotting method and also demonstrate the capability of this strategy to achieve a novel colorimetric
DNA sensor without any complicated process.