In Vivo Chemoprotective Activity of Bovine Dialyzable Leukocyte Extract in Mouse Bone Marrow Cells against Damage Induced by 5-Fluorouracil.

Abstract

Chemotherapy treatments induce a number of side effects, such as leukopenia neutropenia, peripheral erythropenia, and thrombocytopenia, affecting the quality of life for cancer patients. 5-Fluorouracil (5-FU) is wieldy used as myeloablative model in mice. The bovine dialyzable leukocyte extract (bDLE) or IMMUNEPOTENT CRP® (ICRP) is an immunomodulatory compound that has antioxidants and anti-inflammatory effects. In order to investigate the chemoprotection effect of ICRP on bone marrow cells in 5-FU treated mice, total bone marrow (BM) cell count, bone marrow colony forming units-granulocyte/macrophage (CFU-GM), cell cycle, immunophenotypification, ROS/superoxide and Nrf2 by flow cytometry, and histological and hematological analyses were performed. Our results demonstrated that ICRP increased BM cell count and CFU-GM number, arrested BM cells in G0/G1 phase, increased the percentage of leukocyte, granulocytic, and erythroid populations, reduced ROS/superoxide formation and Nrf2 activation, and also improved hematological levels and weight gain in 5-FU treated mice. These results suggest that ICRP has a chemoprotective effect against 5-FU in BM cells that can be used in cancer patients.

• Authors: Erika Evangelina Coronado-Cerda, Moisés Armides Franco-Molina, Edgar Mendoza-Gamboa, Heriberto Prado-García, Lydia Guadalupe Rivera-Morales, Pablo Zapata-Benavides, María Del Carmen Rodríguez-Salazar, Diana Caballero-Hernandez, Reyes Silvestre Tamez-Guerra, Cristina Rodríguez-Padilla
• Journal: Journal of immunology research (J Immunol Res) Vol. 2016 Pg. 6942321 ( 2016) ISSN: 2314-7156 [Electronic] Egypt
• PMID: 27191003 (Publication Type: Journal Article)

Chemical References

• Antimetabolites, Antineoplastic
• Biological Products
• NF-E2-Related Factor 2
• Protective Agents
• Reactive Oxygen Species
• Superoxides
• Fluorouracil

Topics

• Animals
• Antimetabolites, Antineoplastic (pharmacology)
• Biological Products (pharmacology)
• Bone Marrow (drug effects, metabolism, pathology)
• Bone Marrow Cells (drug effects)
• Cattle
• Cell Cycle (drug effects)
• Chemoprevention
• Fluorouracil (pharmacology)
• Hematopoietic Stem Cells
• Leukocyte Count
• Leukocytes (chemistry)
• Male
• Mice
• NF-E2-Related Factor 2 (metabolism)
• Protective Agents (pharmacology)
• Reactive Oxygen Species (metabolism)
• Superoxides (metabolism)