HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Quantitative determination of intracellular Asulacrine in MCF-7 breast cancer cells by liquid chromatography-mass spectrometry and its application to cellular pharmacokinetic studies of P188 modified liposomes.

Abstract
Asulacrine (ASL), an analogue of amsacrine, has shown higher anti-breast and anti-lung cancer activity. Hereby, a new sensitive and selective liquid chromatography-mass spectrometry (LC/MS) method was developed to determine intracellular asulacrine. The chromatographic separation was performed on an Agilent Zorbax Extend-C18 column (2.1 mm i.d. × 50 mm, 5 μm) using gradient elution with water (2 mmol/L ammonium acetate and 0.1% acetic acid) and acetonitrile as the mobile phase. The detection was achieved with selected ion monitoring mode using electrospray ionization in positive mode with target ions at m/z 465.3 and m/z 326.1 for asulacrine and midazolam, respectively. The standard curve showed a good linearity with the lower limit of quantification of 1 ng/mL, as a result of which, the trace concentration of ASL in cell suspension could be quantified. The intra- and inter-day accuracy ranged from -5.28 to 6.5% and from -6.32 to 1.05%, and the intra- and inter-day precisions were no more than 7.65% and 11.71%, respectively. Additionally, no degradation of asulacrine was observed during stability evaluation. The method was proved to be powerful and practical to determine and compare the intracellular distribution and kinetics of ASL under different formulations in MCF-7 breast cancer cells.
AuthorsXiaojie Zang, Jingwei Zhang, Yaqian Zhou, Qianying Chen, Ying Peng, Jianguo Sun, Jiali Liu, Wenyue Liu, Guangji Wang, Fang Zhou
JournalBiomedical chromatography : BMC (Biomed Chromatogr) Vol. 30 Issue 12 Pg. 1908-1914 (Dec 2016) ISSN: 1099-0801 [Electronic] England
PMID27187844 (Publication Type: Journal Article, Validation Study)
CopyrightCopyright © 2016 John Wiley & Sons, Ltd.
Chemical References
  • Antineoplastic Agents
  • Liposomes
  • Amsacrine
  • asulacrine
Topics
  • Amsacrine (analogs & derivatives, pharmacokinetics)
  • Antineoplastic Agents (pharmacokinetics)
  • Breast Neoplasms (metabolism, pathology)
  • Chromatography, Liquid (methods)
  • Female
  • Humans
  • Liposomes
  • MCF-7 Cells
  • Mass Spectrometry (methods)
  • Reproducibility of Results

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: