Karyopherin α2 induces apoptosis in tongue squamous cell carcinoma CAL-27 cells through the p53 pathway.

Abstract

Tumor onset and progression are associated with dysfunction of the nuclear transport machinery at the level of import and export receptors. However, the role of Karyopherin α2 (KPNA2) in human tongue squamous cell carcinoma (TSCC) remains unknown. We assessed the proliferation, apoptosis and migration of TSCC CAL-27 cells using wound healing, Transwell and MTT assays, western blotting, electron microscopy and acridine orange/ethidium bromide staining following knockdown of KPNA2. The results revealed the antiproliferative, proapoptotic and anti-migratory effects of KPNA2 silencing on the TSCC CAL-27 cells. Moreover, the knockdown of KPNA2 proved to be accompanied by the upregulation of active caspase-3, cytochrome c, Bax, Bad and decreased expression of Bcl-2, p-Bad and XIAP. KPNA2 activated the caspase-dependent pathway in the CAL-27 cells with upregulation of p53, p21Cip1/Waf1 and p16INK4a. Thus, the present study demonstrated that p53/p21Cip1/Waf1/p16INK4a may be an important pathway involved in the function of KPNA2 in TSCC CAL-27 cells.

• Authors: Li Gao, Lei Yu, Chun-Ming Li, Ying Li, Bao-Lin Jia, Bin Zhang
• Journal: Oncology reports (Oncol Rep) Vol. 35 Issue 6 Pg. 3357-62 (Jun 2016) ISSN: 1791-2431 [Electronic] Greece
• PMID: 27109484 (Publication Type: Journal Article)

Chemical References

• CDKN1A protein, human
• CDKN2A protein, human
• Cyclin-Dependent Kinase Inhibitor p16
• Cyclin-Dependent Kinase Inhibitor p21
• KPNA2 protein, human
• TP53 protein, human
• Tumor Suppressor Protein p53
• alpha Karyopherins
• Caspases

Topics

• Apoptosis
• Carcinoma, Squamous Cell (pathology)
• Caspases (physiology)
• Cell Line, Tumor
• Cell Movement
• Cell Survival
• Cyclin-Dependent Kinase Inhibitor p16 (physiology)
• Cyclin-Dependent Kinase Inhibitor p21 (physiology)
• Humans
• Signal Transduction (physiology)
• Tongue Neoplasms (pathology)
• Tumor Suppressor Protein p53 (analysis, physiology)
• alpha Karyopherins (physiology)