Detection of Plasmodium falciparum using a cloned DNA probe: a simple procedure suitable for field application.

Abstract

A simple procedure was developed for spotting blood samples directly onto nylon membrane filter, without the necessity to treat samples with pronase or proteinase K, followed by hybridizing with 32P-labelled DNA probe, pUNK1-45. This probe detected specifically P. falciparum DNA and did not cross react with DNA from man, P. knowlesi, P. chabaudi or P. cynomolgi. The probe was sensitive to detect a parasitemia of 0.001% in 20 microliters of blood.

• Authors: V Boonsaeng, K Chansiri, P Vilasineekul, P Wilairat, S Panyim
• Journal: The Southeast Asian journal of tropical medicine and public health (Southeast Asian J Trop Med Public Health) Vol. 20 Issue 4 Pg. 519-22 (Dec 1989) ISSN: 0125-1562 [Print] Thailand
• PMID: 2700843 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Probes
• Phosphorus Radioisotopes

Topics

• Animals
• DNA Probes
• Epidemiologic Methods
• Humans
• Malaria (blood, diagnosis)
• Nucleic Acid Hybridization
• Phosphorus Radioisotopes
• Plasmodium falciparum (isolation & purification)