Expression Depression of CD300LG-γ in Human Pulmonary Carcinoma.

Abstract

The expression of CD300LG-γ at mRNA and protein level was detected by semiquantitative reverse transcription-polymerase chain reaction (PCR), quantitative real-time PCR, and western blot, and compared by t-test analysis. The results of semiquantitative reverse transcription-PCR showed that the mRNA expression of CD300LG-γ was significantly lower in pulmonary carcinoma tissues, compared to tumor-adjacent tissues. The results of quantitative real-time PCR confirmed this finding. The mRNA level of CD300LG-γ in pulmonary carcinoma tissues compared with tumor-adjacent tissues was 0.436-fold in a median. The results of western blot indicated that CD300LG-γ protein was expressed in both pulmonary carcinoma and tumor-adjacent tissues, and the expression level was significantly lower in pulmonary carcinoma tissues compared with tumor-adjacent tissues. Both mRNA and protein levels of CD300LG-γ in pulmonary carcinoma tissues were significantly lower than that in tumor-adjacent tissues, which might lead to inhibition of killing function of immunocytes, resulting in immune escape of lung cancer cells.

• Authors: Songhui Zhai, He Xu, Xiaomei Jiang, Sheng Sun, Lang Qin, Dapeng Wei, Lijuan Hu
• Journal: Monoclonal antibodies in immunodiagnosis and immunotherapy (Monoclon Antib Immunodiagn Immunother) Vol. 35 Issue 2 Pg. 94-9 (Apr 2016) ISSN: 2167-9436 [Electronic] United States
• PMID: 26977771 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• CD300LG protein, human
• RNA, Messenger
• Receptors, Immunologic

Topics

• Antigens, CD (biosynthesis, immunology)
• Carcinoma, Non-Small-Cell Lung (genetics, immunology, pathology)
• Cell Line, Tumor
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (genetics, immunology, pathology)
• Male
• RNA, Messenger (biosynthesis)
• Receptors, Immunologic (biosynthesis, immunology)