Decrease of TET2 expression and increase of 5-hmC levels in myeloid sarcomas.

Abstract

BACKGROUND:

Myeloid sarcoma is a tumor mass that consists of myeloblasts or immature myeloid cells at an extramedullary site. Pathological diagnosis is very difficult based on morphology if systemic signs of disease are absent. The subtype of myeloid sarcoma is also minimally identifiable in the histological picture.

FINDINGS:

We investigated 18 paraffin-embedded myeloid sarcoma samples, and our immunohistochemical data confirmed the relevance of some key markers for the diagnosis and subclassification of myeloid sarcoma. CD34 was found as a marker in 67% of the myeloid sarcoma cases, and CD34 was positive in all immature types of myeloid sarcoma. CD68 was found in 83% of the myeloid sarcoma cases, but CD68 was most identified in the differentiated type of myeloid sarcoma. Myeloperoxidase (MPO) was positive in all myeloid sarcomas. Notably, the reactivity of MPO in the blastic subtype was much lower in myeloid sarcomas. CD117 reactivity was found in 67% of myeloid sarcomas. Ten-eleven translocation 2 (TET2) protein exhibited significant negative reactivity in 88% of the cases, and 5-methylcytosine (5-hmC) was significantly positive in the nucleus in 100% of the cases.

CONCLUSIONS:

Our findings indicated that an immunohistochemical panel that included MPO, CD68 and CD34 could be used for the detection of blastic, differentiated and immature types of myeloid sarcoma. Changes in novel epigenetic regulators, including the loss of TET2 and gain of 5-hmC, as characteristics of myeloid malignancies may be useful novel markers of myeloid sarcoma.

• Authors: Desheng Xiao, Ying Shi, Chunyan Fu, Jiantao Jia, Yu Pan, Yiqun Jiang, Ling Chen, Shuang Liu, Wen Zhou, Jianhua Zhou, Yongguang Tao
• Journal: Leukemia research (Leuk Res) Vol. 42 Pg. 75-9 (Mar 2016) ISSN: 1873-5835 [Electronic] England
• PMID: 26811000 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2016 Elsevier Ltd. All rights reserved.

Chemical References

• 5-hydroxymethyl-2'-deoxycytidine
• Antigens, CD
• Antigens, CD34
• Antigens, Differentiation, Myelomonocytic
• Biomarkers, Tumor
• CD68 antigen, human
• DNA-Binding Proteins
• Interleukin-3
• Proto-Oncogene Proteins
• Recombinant Fusion Proteins
• myelopoietin
• Deoxycytidine
• Granulocyte Colony-Stimulating Factor
• Formaldehyde
• 5-Methylcytosine
• Dioxygenases
• TET2 protein, human

Topics

• 5-Methylcytosine (analysis, biosynthesis)
• Adult
• Antigens, CD (analysis, biosynthesis)
• Antigens, CD34 (analysis, biosynthesis)
• Antigens, Differentiation, Myelomonocytic (analysis, biosynthesis)
• Biomarkers, Tumor (analysis)
• DNA-Binding Proteins (analysis, biosynthesis)
• Deoxycytidine (analogs & derivatives, analysis, biosynthesis)
• Dioxygenases
• Female
• Formaldehyde
• Granulocyte Colony-Stimulating Factor (analysis, biosynthesis)
• Humans
• Immunohistochemistry
• Interleukin-3 (analysis, biosynthesis)
• Male
• Middle Aged
• Paraffin Embedding
• Proto-Oncogene Proteins (analysis, biosynthesis)
• Recombinant Fusion Proteins (analysis, biosynthesis)
• Sarcoma, Myeloid (diagnosis)
• Tissue Fixation
• Young Adult