Abstract |
OM-X® is a hand-made and naturally manufactured probiotic supplement. This fermented food product is made from vegetables, fruits, seaweeds and mushrooms, using 12 strains of lactic acid bacteria and bifidobacteria. OM-X® is also known to have beneficial health properties, and some of its components show effects on antigen (Ag)-stimulated degranulation activity, indicating that OM-X® may be useful in the treatment of allergy responses and symptoms. In this study, we evaluated the inhibitory effects of OM-X® on Ag-stimulated degranulation in rat basophilic leukemia RBL-2H3 cells, clarified the underlying mechanisms, and determined the active compounds in OM-X® for suppression of degranulation. Treatment with OM-X® gradually suppressed Ag-stimulated degranulation throughout the maturation period. OM-X® also gradually produced melanoidins by lactic acid bacterial fermentation during the maturation process. There was a high correlation between the suppression levels of Ag-stimulated degranulation and the browning of OM-X®. Furthermore, the inhibition of Ag-stimulated degranulation by OM-X® was found to be partially due to the direct inactivation of NADPH oxidase. To elucidate the in vivo effects of OM- X®, type I allergy model mice were orally administered with OM-X®, and the passive cutaneous anaphylaxis (PCA) reaction was measured. OM-X® intake remarkably suppressed the PCA reaction. Taken together, our findings suggest that OMX® could be a beneficial food to ameliorate allergic reactions.
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Authors | Tomohiro Itoh, Yasuyoshi Miyake, Takuya Kasashima, Yoshie Shimomiya, Yuki Nakamura, Masashi Ando, Yasuyuki Tsukamasa, Muneaki Takahata |
Journal | Natural product communications
(Nat Prod Commun)
Vol. 10
Issue 9
Pg. 1597-601
(Sep 2015)
ISSN: 1934-578X [Print] United States |
PMID | 26594768
(Publication Type: Journal Article)
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Chemical References |
- Biphenyl Compounds
- Free Radical Scavengers
- Picrates
- Reactive Oxygen Species
- 1,1-diphenyl-2-picrylhydrazyl
- Calcium
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Topics |
- Animals
- Biphenyl Compounds
- Calcium
(metabolism)
- Cell Line, Tumor
- Fermentation
- Free Radical Scavengers
- Leukemia, Basophilic, Acute
(metabolism)
- Male
- Mice
- Mice, Inbred ICR
- Passive Cutaneous Anaphylaxis
(drug effects)
- Picrates
- Rats
- Reactive Oxygen Species
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