The aim of this experimental study was to investigate the effects of low-intensity light-emitting diode (LED)
phototherapy on the inflammatory process in the calcaneal tendon of ovariectomized rats (OVX) through the involvement of the inflammatory mediators
interleukin (IL)-6,
IL-10, and
tumor necrosis factor-alpha (TNF-α). Thirty-five female Wistar rats were divided into 4 groups: 3 groups of OVX rats totaling 30 rats (untreated OVX rats [OVX injury group], treated OVX rats [OVX LED group], and control OVX rats; subgroups existed based on the sampling times, which were 3, 7, and 14 days) and 1 group of non-OVX rats (not OVX; n = 5).
Tendon injury was induced by
trauma using a
208-g mass placed at 20 cm from the right tendon of each animal with energy of 0.70 J. The animals were treated 12 h after
tendonitis with LED
therapy and every 48 h thereafter until
euthanasia (at 3, 7, or 14 days). The tendons were dissected and stored in liquid
nitrogen at -196 °C, thawed only at the time of immunoenzymatic testing (ELISA). Groups treated with LED showed a decrease in the number of pro-inflammatory cells,
IL-6, and TNF-α (p <0.05), and an increase in
IL-10 (p < 0.05) when compared to the not OVX group (p < 0.05). It was concluded that low-intensity LED treatment using the parameters and wavelength of 945 nm in the time periods studied reduced the release of
IL-6 and TNF-α and increased the release of
IL-10, thereby improving the inflammatory response in OVX rats.