Mmu-miR-351 attenuates the survival of cardiac arterial endothelial cells through targeting STAT3 in the atherosclerotic mice.

Abstract

The signal transducer and activator of transcription 3 (STAT3) signaling pathway was involved in regulation of endothelial cell survival/apoptosis and was regarded as a target for prevention of atherosclerosis or other cardiovascular diseases. Factors, regulating STAT3 expression and activity, have aroused a wide range of interest, such as miRNAs or transcription factors. The aim of this study is to explore the role of miR-351, a miRNA found not long before, in the regulation of STAT3 expression and endothelial cell survival in the model mice with atherosclerosis (AS). Expression of miR-351 in the serum and cardiac arterial endothelial cells of the WT mice and AS mice was detected. Real-time qPCR analysis showed that miR-351 was upregulated in the serum and endothelial cells of the AS mice, displaying an opposite expression pattern with STAT3. To explore the role and mechanism of miR-351 in the endothelial cell survival, the miR-351 mimic was transfected in to the endothelial cells. MTT and Trypan Blue assays showed miR-351 attenuated the survival of endothelial cells. Our results of the TargetScan output and the 3'UTR luciferase reporter assay indicated that STAT3 was target of miR-351. Additionally, miR-351 resisted the elevation of STAT3 protein level and promotion of endothelial cell survival caused by SD19. Finally, our in vitro angiogenesis assay revealed that miR-351 suppressed angiogenesis and resisted the promotion of angiogenesis caused by SD19. In conclusion, miR-351 was upregulated in the atherosclerotic mice. MiR-351 can attenuate the survival of endothelial cells and suppress angiogenesis through targeting STAT3 in vitro.

• Authors: Ying Zhang, Yujie Liu, Hong Zhang, Minghui Wang, Jinlian Zhang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) 2015 Dec 4-11 Vol. 468 Issue 1-2 Pg. 300-5 ISSN: 1090-2104 [Electronic] United States
• PMID: 26505789 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• MIRN351 microRNA, 351
• MicroRNAs
• STAT3 Transcription Factor
• Janus Kinase 2

Topics

• Animals
• Atherosclerosis (genetics, metabolism, pathology)
• Cell Survival
• Cells, Cultured
• Coronary Vessels (cytology, metabolism, pathology)
• Endothelium, Vascular (metabolism, pathology)
• Gene Expression Regulation
• Janus Kinase 2 (metabolism)
• Mice
• MicroRNAs (genetics)
• Neovascularization, Pathologic (genetics, metabolism, pathology)
• Oxidative Stress
• STAT3 Transcription Factor (genetics, metabolism)
• Signal Transduction