Dengue virus infection induces formation of G3BP1 granules in human lung epithelial cells.
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| Abstract |
Cells reprogram ongoing translation in response to viral infection, resulting in formation of stress granules (SGs), while viruses have evolved a variety of strategies to antagonize the host SG response. Previous literature reported that in BHK-1 cells, infection with dengue virus (DENV) interfered with the SG formation. In the current study, we further investigated SG formation in human epithelial A549 cells by detecting subcellular localization of two SG hallmarks, TIA-1 and G3BP1. In response to DENV type 2 (DENV2) and type 3 (DENV3) infection, G3BP1, but not TIA-1, was recruited into cytoplasmic granules in some cells, and viral protein synthesis was significantly impaired in the G3BP1-granule-containing cells. Knockdown of G3BP1 significantly rescued the dsRNA-mediated suppression of DENV2 replication. Furthermore, our data showed that the phosphorylation of protein kinase regulated by dsRNA (PKR) and eIF2α, as well as accumulation of dsRNA, mainly occurred at the late stage of viral infection. This work revealed that in DENV-infected A549 cells, G3BP1 granules were assembled independently of TIA-1 and had a negative impact on viral replication. This extends our understanding of the antagonistic relationship between the SG response and dengue virus infection.
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| Authors | Jun Xia, Xiaoyan Chen, Feng Xu, Yi Wang, Yongxia Shi, Yuye Li, Junfang He, Ping Zhang |
| Journal | Archives of virology (Arch Virol) Vol. 160 Issue 12 Pg. 2991-9 (Dec 2015) ISSN: 1432-8798 [Electronic] Austria |
| PMID | 26350772 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
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