Abstract | OBJECTIVES:
Osteosarcoma is the most common type of malignant bone tumour in children and adolescents; it has poor prognosis, is highly metastatic and is resistant to current therapeutic approaches. In this study, different herbal extracts used in phytotherapy have been screened after searching innovative natural anti- cancer components. MATERIALS AND METHODS: Twenty steroid glycosides were examined for accordance to their potential of inhibiting cell proliferation and inducing apoptosis in the osteosarcoma cell line 143B. Cell proliferation was examined using a CASY counter. Effects of cardiac glycosides on induction of apoptosis were evaluated by Annexin V-APC and flow cytometry, caspase activity assay and measurement of mitochondrial membrane potential. RESULTS: CONCLUSION:
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Authors | C I Delebinski, S Georgi, S Kleinsimon, M Twardziok, B Kopp, M F Melzig, G Seifert |
Journal | Cell proliferation
(Cell Prolif)
Vol. 48
Issue 5
Pg. 600-10
(Oct 2015)
ISSN: 1365-2184 [Electronic] England |
PMID | 26300346
(Publication Type: Journal Article)
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Copyright | © 2015 John Wiley & Sons Ltd. |
Chemical References |
- Glycosides
- Steroids
- Caspase 8
- Caspase 9
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Topics |
- Apoptosis
(drug effects)
- Bone Neoplasms
(metabolism, pathology)
- Caspase 8
(metabolism)
- Caspase 9
(metabolism)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Glycosides
(chemistry, pharmacology)
- Humans
- Membrane Potential, Mitochondrial
(drug effects)
- Osteosarcoma
(metabolism, pathology)
- Steroids
(chemistry)
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