Role of the tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) from the hard tick Haemaphysalis longicornis in immunity against bacterial infection.

Abstract

A tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) is a negative feedback regulator that controls excessive immune responses in vertebrates. The sequence of tick hemolymph TRAFD1 from the hard tick Haemaphysalis longicornis (HlTRAFD1) was analyzed after identification and cloning from the expressed sequence tag database. RT-PCR and Western blot analyses showed that HlTRAFD1 transcript and protein levels after blood feeding were present in all developmental stages, and the transcript level was consistently high in all organs examined from adult female ticks upon engorgement. Knockdown of HlTRAFD1 gene by RNA interference did not affect blood feeding or oviposition. However, HlTRAFD1 silencing affected the expression of the longicin gene, a defensin-like molecule, but not the lysozyme gene. Moreover, the survival rate of HlTRAFD1-silenced ticks was lower, and the number of E. coli was higher in the hemolymph and plasmatocytes after E. coli injection compared to the control group. These results suggested that HlTRAFD1 strongly affected both the humoral and cellular immunity of ticks.

• Authors: Rie Takechi, Remil Linggatong Galay, Tomohide Matsuo, Hiroki Maeda, Kodai Kusakisako, Melbourne Rio Talactac, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
• Journal: Ticks and tick-borne diseases (Ticks Tick Borne Dis) Vol. 7 Issue 1 Pg. 36-45 (Feb 2016) ISSN: 1877-9603 [Electronic] Netherlands
• PMID: 26283173 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier GmbH. All rights reserved.

Chemical References

• Intracellular Signaling Peptides and Proteins
• Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
• enhanced green fluorescent protein
• longicin
• Green Fluorescent Proteins
• 4-Butyrolactone

Topics

• 4-Butyrolactone (analogs & derivatives, genetics, metabolism)
• Animals
• Cloning, Molecular
• Escherichia coli (metabolism, physiology)
• Feeding Behavior
• Female
• Gene Expression Regulation (immunology)
• Green Fluorescent Proteins (metabolism)
• Hemocytes (metabolism, microbiology)
• Host-Pathogen Interactions
• Intracellular Signaling Peptides and Proteins (genetics, metabolism)
• Ixodidae (genetics, immunology, metabolism, microbiology)
• Mice
• RNA Interference
• Rabbits
• Reproduction
• Tumor Necrosis Factor Receptor-Associated Peptides and Proteins (genetics, metabolism)
• Zinc Fingers