[Immunoelectron microscopic observation of membranous nephropathy in children using protein A-gold complex method].

Abstract

The ultrastructural localization of immunoglobulins (IgG, IgA, IgM), complement component (C3c), or fibrinogen-related antigen (FRA) was investigated on 5 biopsy samples from 5 children with idiopathic MN or lupus nephritis using protein A-gold (PAG) complex method. The immunoreactivity of IgG was essentially confined to the mesangial and subepithelial electron-dense deposit (EDD) in all of 5 children, and more intense staining of IgG was observed in 3 children with stage II of MN than those with stage I and stage III of MN. Double immunocytochemical staining showed the same distribution of both IgG and IgM or both IgG and FRA in subepithelial EDD in 2 of 5 children. These findings suggest that IgG deposits are associated with the formation of subepithelial EDD in MN, and raise the possibility that IgM and FRA deposits may result from entrapment and/or immunological reaction.

• Authors: S Kawahara, T Matsunaga, S Sakamoto, K Taira, H Kamitsuji, M Nakajima
• Journal: Nihon Jinzo Gakkai shi (Nihon Jinzo Gakkai Shi) Vol. 31 Issue 11 Pg. 1143-9 (Nov 1989) ISSN: 0385-2385 [Print] Japan
• PMID: 2625738 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antigen-Antibody Complex
• Antigens
• Immunoglobulin G
• Staphylococcal Protein A
• fibrinogen-related antigen
• Gold
• Complement C3c

Topics

• Adolescent
• Antigen-Antibody Complex (analysis)
• Antigens (analysis)
• Child
• Complement C3c (analysis)
• Female
• Glomerulonephritis, Membranous (immunology, metabolism)
• Gold
• Humans
• Immunoglobulin G (analysis)
• Immunohistochemistry (methods)
• Kidney (immunology)
• Lupus Nephritis (immunology, metabolism)
• Male
• Staphylococcal Protein A