Synergic carcinostatic effects of ascorbic acid and hyperthermia on Ehrlich ascites tumor cell.

Abstract

AIM:

In this study, we evaluated the carcinostatic effects of combined ascorbic acid (AsA) and a capacitive-resistive electric transfer (CRet) hyperthermic apparatus-induced hyperthermic treatment on Ehrlich ascites tumor (EAT) cells.

MATERIALS AND METHODS:

EAT cells were exposed to various AsA (0-10 mM) concentrations for 1 h; they subsequently underwent CRet treatment for 15 min at 42 °C. Cell viability was assessed by the WST-8 assay 24 h after the combined treatment. Reactive oxygen species involvement was evaluated using catalase and tempol; caspase-3/7 activation was determined by their fluorescent substrates; cell proliferation were estimated by time-lapse observation. The effect on the cell cycle was analyzed by flow cytometry.

RESULTS:

Combined AsA and CRet treatment synergistically suppressed cell viability compared with either treatment alone, and these synergistically carcinostatic effects were evident even at noncytotoxic concentrations of AsA alone (≤ 2 mM). The carcinostatic effects of combined AsA and CRet treatment were attenuated in a dose-dependent manner by catalase addition, but not by the superoxide anion radical scavenger tempol. Time-lapse observation revealed that combined AsA and CRet treatment activated caspase-3/7 at 10-24 h after treatment, accompanied by significant cell growth suppression. Cell cycle analysis revealed that the rate of sub-G1-phase (apoptotic) cells was drastically increased at 12 h and 24 h, and that the G2/M-phase cells gradually increased at 6-24 h after treatment.

CONCLUSION:

These results indicate that combined AsA and CRet treatment synergistically inhibits EAT cell growth through G2/M arrest and apoptosis induction via H2O2 generation at lower AsA concentrations; this carcinostatic effect cannot be exerted by AsA alone.

• Authors: Y Saitoh, T Yoshimoto, S Kato, N Miwa
• Journal: Experimental oncology (Exp Oncol) Vol. 37 Issue 2 Pg. 94-9 (Jun 2015) ISSN: 2312-8852 [Electronic] Ukraine
• PMID: 26112934 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Reactive Oxygen Species
• Ascorbic Acid

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis
• Ascorbic Acid (pharmacology)
• Carcinoma, Ehrlich Tumor (therapy)
• Cell Cycle Checkpoints
• Cell Line, Tumor
• Cell Proliferation
• Cell Survival
• Combined Modality Therapy
• Drug Screening Assays, Antitumor
• Hyperthermia, Induced
• Reactive Oxygen Species (metabolism)