Abstract | BACKGROUND: METHODS: We investigated the cytosolic proteins produced by irradiated tumor cells by using a quantitative proteomic approach based on stable isotope labeling by amino acids in cell culture. MDA-MB-231 breast cancer cells were treated with a single or fractionated 10 Gray dose of (137)Cs γ-radiation, which was selected based on cell viability. RESULTS: Radiation-induced proteins were differentially expressed based on the fractionated times of radiation and were involved in multiple biological functions, including energy metabolism and cytoskeleton organization. We identified 46 proteins increased by at least 1.3-fold, and high ranks were determined for cathepsin D, gelsolin, arginino- succinate synthase 1, peroxiredoxin 5, and C-type mannose receptor 2. CONCLUSION: These results suggest that a number of tumor-derived factors upregulated by γ-radiation are promising targets for modulation of the immune response during radiation treatment.
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Authors | Mi-Hyoung Kim, Seung-Youn Jung, Jiyeon Ahn, Sang-Gu Hwang, Hee-Jong Woo, Sungkwan An, Seon Young Nam, Dae-Seog Lim, Jie-Young Song |
Journal | Cell & bioscience
(Cell Biosci)
Vol. 5
Pg. 2
( 2015)
ISSN: 2045-3701 [Print] England |
PMID | 26056562
(Publication Type: Journal Article)
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