Cardiac and renal nitrosative-oxidative stress after acute poisoning by a nerve agent Tabun.

Abstract

We hypothesized that Tabun poisoning, as well as other organophosphorous treatment, cause specific organs' oxidative changes that have not previously been substantiated investigated. In this regard, a marker for nitrosative-oxidative stress in the main haemodynamic organs (heart and kidney) could reveal the existence of such changes. In this study, for the first time we studied the nitrosative/oxidative stress in heart and kidney after acute Tabun (Ethyl N,N- Dimethylphosphoramidocyanidate) poisoning measuring by immunohistochemistry the expression of 3-nitrotyrosine--a marker for nitrosative-oxidative stress. We investigated nitrotyrozine expression in three different groups of animals (with at least 3 animals in each group): the first group was treated with 0.5 LD50 Tabun and organs were collected after 24 h; the second group received vehicle for the same period; in the third group a highly specific re-activator was applied immediately after Tabun application. Heart and kidney were collected after 24 h. The levels of nitrotyrozine production significantly increased (more than 3 times) in cardiomyocytes after Tabun. The application of re-activator slightly reduced these levels not reaching the basal heart levels. Nitrotyrozine expression in kidney increased more than 2 times after Tabun and application of re-activator did not change it significantly. In conclusion, our study evidently demonstrated that Tabun trigger oxidative-nitrosative stress in heart and kidney and these cellular effects should be protected by an additional anti-oxidant therapy, since acetylcholinesterase re-activator is not efficient in this manner.

• Authors: Dimo Dimov, Radka Hadjiolova, Kamen Kanev, Radka Tomova, Anna Michova, Todor Todorov, Rumen Murdjev, Temenujka Boneva, Ivanka Dimova
• Journal: Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering (J Environ Sci Health A Tox Hazard Subst Environ Eng) Vol. 50 Issue 8 Pg. 824-9 ( 2015) ISSN: 1532-4117 [Electronic] England
• PMID: 26030688 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nerve Agents
• Organophosphates
• 3-nitrotyrosine
• Tyrosine
• tabun

Topics

• Animals
• Heart (drug effects)
• Kidney (drug effects, metabolism)
• Male
• Myocardium (metabolism)
• Nerve Agents (toxicity)
• Organophosphates (toxicity)
• Oxidation-Reduction (drug effects)
• Oxidative Stress (drug effects)
• Rats
• Rats, Wistar
• Tyrosine (analogs & derivatives)