Abstract | BACKGROUND: METHODS: Murine MN was experimentally induced by daily subcutaneous administration of cationic bovine serum albumin, with phosphate-buffered saline used in control mice. MN mice were untreated or given RSV. Disease severity and pathogenesis was assessed by determination of metabolic and histopathology profiles, lymphocyte subsets, immunoglobulin production, oxidative stress, apoptosis, and production of heme oxygenase-1 (HO1). RESULTS: MN mice given RSV had significantly reduced proteinuria and a marked amelioration of glomerular lesions. RSV also significantly attenuated immunofluorescent staining of C3, although there were no changes of serum immunoglobulin levels or immunocomplex deposition in the kidneys. RSV treatment of MN mice also reduced the production of reactive oxygen species (ROS), reduced cell apoptosis, and upregulated heme oxygenase 1 (HO1). Inhibition of HO1 with tin protoporphyrin IX partially reversed the renoprotective effects of RSV. The HO1 induced by RSV maybe via Nrf2 signaling. CONCLUSION: Our results show that RSV increased the expression of HO1 and ameliorated the effects of membranous nephropathy in a mouse model due to its anti- complement, anti-oxidative, and anti-apoptotic effects. RSV appears to have potential as a treatment for MN.
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Authors | Chia-Chao Wu, Yen-Sung Huang, Jin-Shuen Chen, Ching-Feng Huang, Sui-Lung Su, Kuo-Cheng Lu, Yuh-Feng Lin, Pauling Chu, Shih-Hua Lin, Huey-Kang Sytwu |
Journal | PloS one
(PLoS One)
Vol. 10
Issue 5
Pg. e0125726
( 2015)
ISSN: 1932-6203 [Electronic] United States |
PMID | 25954969
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Metalloporphyrins
- Protoporphyrins
- Reactive Oxygen Species
- Serum Albumin
- Stilbenes
- Complement System Proteins
- tin protoporphyrin IX
- Heme Oxygenase-1
- Resveratrol
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Topics |
- Animals
- Apoptosis
(drug effects)
- Cattle
- Complement System Proteins
(drug effects)
- Disease Models, Animal
- Glomerulonephritis, Membranous
(drug therapy, genetics, pathology)
- Heme Oxygenase-1
(biosynthesis, genetics)
- Kidney
(drug effects, injuries, metabolism)
- Kidney Glomerulus
(drug effects, metabolism)
- Metalloporphyrins
(administration & dosage)
- Mice
- Oxidative Stress
(drug effects)
- Protoporphyrins
(administration & dosage)
- Reactive Oxygen Species
(metabolism)
- Resveratrol
- Serum Albumin
(administration & dosage)
- Stilbenes
(administration & dosage)
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