MiR-31 Downregulation Protects Against Cardiac Ischemia/Reperfusion Injury by Targeting Protein Kinase C Epsilon (PKCε) Directly.

Abstract

BACKGROUND:

Various miRNAs have been shown to participate in cardiac ischemia/reperfusion injury (I/R). miR-31 was identified as the most strikingly upregulated miRNA after acute myocardial infarction; therefore, the underlying role and mechanism of miR-31 in cardiac I/R was investigated.

METHODS:

miR-31 expression was detected after cardiac I/R in mice. The cardioprotective effect of miR-31 downregulation was assessed in vitro and in vivo. The functional target gene and its downstream molecule were determined.

RESULTS:

miR-31 expression increased after I/R. miR-31 downregulation increased cell viability and SOD activity and decreased LDH activity and MDA content in vitro. Additionally, miR-31 downregulation alleviated myocardial infarct size in vivo. PKCε was identified as the functional target gene of miR-31, and NFκB was identified as its downstream molecule that was involved in the miR-31-mediated cardioprotective effect.

CONCLUSION:

miR-31 expression increased throughout the cardiac I/R process, and miR-31 downregulation induced a cardioprotective effect via a miR-31/PKCε/NFκB-dependent pathway.

• Authors: Yongyi Wang, Min Men, Wengang Yang, Hui Zheng, Song Xue
• Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (Cell Physiol Biochem) Vol. 36 Issue 1 Pg. 179-90 ( 2015) ISSN: 1421-9778 [Electronic] Germany
• PMID: 25925791 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• MicroRNAs
• Mirn31 microRNA, mouse
• NF-kappa B
• Lactate Dehydrogenases
• Superoxide Dismutase
• Prkce protein, mouse
• Protein Kinase C-epsilon

Topics

• Animals
• Cell Survival
• Cells, Cultured
• Disease Models, Animal
• Down-Regulation
• Genetic Therapy
• Lactate Dehydrogenases (metabolism)
• Mice
• MicroRNAs (antagonists & inhibitors, genetics, metabolism)
• Myocardial Ischemia (complications, metabolism, pathology)
• Myocardial Reperfusion Injury (metabolism, prevention & control)
• NF-kappa B (metabolism)
• Protein Kinase C-epsilon (genetics, metabolism)
• Signal Transduction
• Superoxide Dismutase (metabolism)