Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V.

Abstract

α-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid α2,6-linked to GalNAc residue that was isolated as a minor compound from the brain. The sialyltransferase ST6GalNAc V was cloned from mouse brain and showed α2,6-sialyltransferase activity almost exclusively for GM1b, to form GD1α and is considered as the main enzyme involved in the biosynthesis of α-series gangliosides. Recently, ST6GALNAC5 was identified as one of the genes over-expressed in breast cancer cell populations selected for their ability to produce brain metastasis. However, the capacity of human breast cancer cells to produce α-series gangliosides has never been clearly demonstrated. Here, we show by stable transfection and MS-MS analysis of total glycosphingolipids that ST6GALNAC5 expressing MDA-MB-231 breast cancer cells accumulate GD1α ganglioside (IV3Neu5Ac1, III6Neu5Ac1Gg4-Cer).

• Authors: Sandy Vandermeersch, Jorick Vanbeselaere, Clément P Delannoy, Aurore Drolez, Caroline Mysiorek, Yann Guérardel, Philippe Delannoy, Sylvain Julien
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 20 Issue 4 Pg. 6913-24 (Apr 16 2015) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 25913930 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ganglioside GD1alpha
• G(M1) Ganglioside
• Sialyltransferases
• brain-specific GD1alpha synthase, ST6GalNAc V

Topics

• Breast Neoplasms (genetics, metabolism)
• Cell Line, Tumor
• Female
• G(M1) Ganglioside (analogs & derivatives, metabolism)
• Humans
• Mass Spectrometry (methods)
• Sialyltransferases (genetics, metabolism)