Strophalloside induces apoptosis of SGC-7901 cells through the mitochondrion-dependent caspase-3 pathway.

Abstract

Cardenolides with special chemical structures have been considered as effective anti-cancer drugs in clinic trials. Strophalloside is a cardenolide we recently isolated from Antiaris toxicaria obtained from Hainan, China. The aim of this study was to investigate the possible anticancer effects induced by strophalloside and the underlying molecular mechanism. Gastric carcinoma SGC-7901 cells were treated with strophalloside at various concentrations for different times, and resulting cell viability was determined by the MTT assay, and the motility and invasion of tumor cells were assessed by the Transwell chamber assay. Apoptosis were measured by Annexin V-FITC/PI and Hoechst staining. The changes of mitochondrial transmembrane potential were examined by a JC-1 kit. The expressions of pro-apoptotic protein cytochrome c, caspase-3 and caspase-9 were detected by western blotting analysis. The results showed that strophalloside was capable of reducing cell viability, inhibiting cell growth, and suppressing cell migration and invasion in a time- and dose-dependent manner. Mitochondrial membrane potential declined and the concentration of cytochrome c increased in cytoplasm and caspase-3 and caspase-9 were cleaved into activated states, suggesting that cytochrome c was released from the mitochondrion to cytoplasm and finally activated the caspase-dependent apoptosis pathway. Our results indicate that strophalloside is a potential anticancer drug.

• Authors: Xue-Jiao Zhang, Wen-Li Mei, Guang-Hong Tan, Cai-Chun Wang, Song-Lin Zhou, Feng-Ru Huang, Bin Chen, Hao-Fu Dai, Feng-Ying Huang
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 20 Issue 4 Pg. 5714-28 (Mar 31 2015) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 25838173 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Cardenolides
• strophalloside
• Caspase 3
• Caspase 9

Topics

• Antineoplastic Agents (administration & dosage)
• Apoptosis (drug effects)
• Cardenolides (administration & dosage, chemistry)
• Caspase 3 (biosynthesis, metabolism)
• Caspase 9 (biosynthesis)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Drug Screening Assays, Antitumor
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Membrane Potential, Mitochondrial (drug effects)
• Mitochondria (drug effects)
• Signal Transduction (drug effects)
• Stomach Neoplasms (drug therapy, pathology)