Effects of N-Butylphthalide on the expressions of Nogo/NgR in rat brain tissue after carbon monoxide poisoning.

Abstract

Carbon monoxide (CO) intoxication is one of the most common types of poisoning worldwide, and may result in neuropathologic sequelae, yet its pathogenesis is not clear and there is no optimal management strategy for patients with CO poisoning. In this study, the rat model of CO poisoning was established in a hyperbaric chamber by CO exposure. Rats were administered orally N-Butylphthalide (NBP) at a dose of 1 ml/100g. Neuronal apoptosis was assessed by TUNEL stain and flow cytometry. The expressions of neurite outgrowth inhibitor (Nogo), myelin-associated glycoprotein (MAG) and Nogo receptor-1 (NgR1) were observed in rat brain tissue by immunohistochemistry and double immunofluorescence staining. As we expected, CO poisoning could start the mechanism of apoptosis. The number of apoptotic cells and the early neuronal apoptosis percentage (EAR) were significantly increased at 1 day, 3 day after CO exposure. NBP treatment obviously reduce neuronal apoptosis and the EAR (P<0.05). CO poisoning could induce Nogo, MAG and NgR1 expressions. The increased Nogo, MAG and NgR1 proteins were still observed at 4 week after CO poisoning. NBP could significantly reduce the levels of Nogo and NgR1 proteins. Then we suspected that the expressions of Nogo, MAG and NGR1 proteins might be associated with brain injury and demyelination induced by CO poisoning. NBP might inhibit neuronal apoptosis and the EAR, down-regulate the expressions of Nogo and NgR1 proteins (but not MAG), and play a neuro-protective role in brain damage after acute CO poisoning.

• Authors: Qin Li, Yongmei Cheng, Ming Jun Bi, Hai Kang, Yun Qu, Hongyang Lin, Yunliang Guo, Yong Zou
• Journal: Environmental toxicology and pharmacology (Environ Toxicol Pharmacol) Vol. 39 Issue 2 Pg. 953-61 (Mar 2015) ISSN: 1872-7077 [Electronic] Netherlands
• PMID: 25812770 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Benzofurans
• GPI-Linked Proteins
• Myelin Proteins
• Myelin-Associated Glycoprotein
• Neuroprotective Agents
• Nogo Proteins
• Nogo Receptor 1
• Receptors, Cell Surface
• Rtn4 protein, rat
• Rtn4r protein, rat
• 3-n-butylphthalide

Topics

• Animals
• Apoptosis (drug effects)
• Benzofurans (pharmacology, therapeutic use)
• Brain (drug effects, metabolism, pathology)
• Carbon Monoxide Poisoning (drug therapy, metabolism)
• GPI-Linked Proteins (metabolism)
• Male
• Myelin Proteins (metabolism)
• Myelin-Associated Glycoprotein (metabolism)
• Neuroprotective Agents (pharmacology, therapeutic use)
• Nogo Proteins
• Nogo Receptor 1
• Rats, Sprague-Dawley
• Receptors, Cell Surface (metabolism)