Association between polymorphisms of interleukin 10 with inflammatory biomarkers in East Chinese Han patients with rheumatoid arthritis.

Abstract

OBJECTIVES:

The aim of the present study was to examine the association between polymorphisms of IL-10 with inflammatory biomarkers in East Chinese Han patients with rheumatoid arthritis (RA).

METHODS:

We examined IL-10 rs1800872 A/C polymorphisms in 615 RA patients, and 839 controls, in an East Chinese Han population. Genotyping was performed using a custom-by-design 48-Plex SNP scan TM Kit. The blood plasma concentration of IL-10 was measured using an Iodine [(125)I] IL-10 Radioimmunoassay Kit, in 90 RA patients and 90 controls.

RESULTS:

IL-10 rs1800872 A/C polymorphisms were associated with risk of RA. Following stratified analysis, an increased risk of RA was associated with the CC genotype among male, older, C-reactive protein-positive, anti-cyclic citrullinated peptide antibody-positive, and rheumatoid factor-positive-patients, and among patients with a DAS28 of≥3.20 or an erythrocyte sedimentation rate of≥25, and in functional class I and II patients. The average plasma concentration of IL-10 was significantly higher in RA patients compared with controls. RA patients positive for the homozygote CC were characterized by significantly higher levels of IL-10 compared with patients with the heterozygote AC. We also found that there were significant relationships between the single nucleotide polymorphisms in the human IL-10 rs1800872 A/C and production of IL-10.

CONCLUSIONS:

Our results suggest that the IL-10 rs1800872 A/C allele might increase the risk of RA. The IL-10 rs1800872 A/C allele might also impact the inflammatory reaction of IL-10 in patients with RA.

• Authors: Ling Ge, Yong Huang, Hui Zhang, Ruiping Liu, Nanwei Xu
• Journal: Joint bone spine (Joint Bone Spine) Vol. 82 Issue 3 Pg. 182-6 (May 2015) ISSN: 1778-7254 [Electronic] France
• PMID: 25623518 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Société française de rhumatologie. Published by Elsevier SAS. All rights reserved.

Chemical References

• Biomarkers
• IL10 protein, human
• Interleukin-10

Topics

• Adult
• Aged
• Alleles
• Arthritis, Rheumatoid (blood, ethnology, genetics)
• Asian People (genetics)
• Biomarkers
• Female
• Genotyping Techniques
• Humans
• Interleukin-10 (blood, genetics)
• Male
• Middle Aged
• Polymorphism, Single Nucleotide