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Probes to monitor activity of the paracaspase MALT1.

Abstract
The human paracaspase mucosa-associated lymphoid tissue lymphoma translocation protein 1 (MALT1) plays a central role in nuclear factor-κB (NF-κB) signaling as both a protease and scaffolding protein. Knocking out MALT1 leads to impaired NF-κB signaling and failure to mount an effective immune response. However, it is unclear to which degree it is the scaffolding function versus the proteolytic activity of MALT1 that is essential. Previous work involving a MALT1 inhibitor with low selectivity suggests that the enzymatic function plays an important role in different cell lines. To help elucidate this proteolytic role of MALT1, we have designed activity-based probes that inhibit its proteolytic activity. The probes selectively label active enzyme and can be used to inhibit MALT1 and trace its activity profile, helping to create a better picture of the significance of the proteolytic function of MALT1.
AuthorsJanna Hachmann, Laura E Edgington-Mitchell, Marcin Poreba, Laura E Sanman, Marcin Drag, Matthew Bogyo, Guy S Salvesen
JournalChemistry & biology (Chem Biol) Vol. 22 Issue 1 Pg. 139-47 (Jan 22 2015) ISSN: 1879-1301 [Electronic] United States
PMID25556944 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier Ltd. All rights reserved.
Chemical References
  • Molecular Probes
  • NF-kappa B
  • Neoplasm Proteins
  • Recombinant Proteins
  • Caspases
  • MALT1 protein, human
  • Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
Topics
  • Caspases (chemistry, genetics, metabolism)
  • Cell Line, Tumor
  • HEK293 Cells
  • Humans
  • Kinetics
  • Molecular Probes (chemistry, metabolism)
  • Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein
  • Mutation
  • NF-kappa B (metabolism)
  • Neoplasm Proteins (chemistry, genetics, metabolism)
  • Protein Binding
  • Proteolysis
  • Recombinant Proteins (biosynthesis, chemistry, genetics)
  • Signal Transduction
  • Transfection

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