Increased production of intestinal immunoglobulins in Syntenin-1-deficient mice.

Abstract

Syntenin-1 is an intracellular PDZ protein that binds multiple proteins and regulates protein trafficking, cancer metastasis, exosome production, synaptic formation, and IL-5 signaling. However, the functions of Syntenin-1 have not yet been clearly characterized in detail, especially in vivo. In this study, we generated a Syntenin-1 knock out (KO) mouse strain and analyzed the role(s) of Syntenin-1 in IL-5 signaling, because the direct interaction of Syntenin-1 with the cytoplasmic domain of the IL-5 receptor α subunit and the regulation of IL-5 signaling by Syntenin-1 have been reported. Unexpectedly, the number of IL-5-responding cells was normal and the levels of fecal immunoglobulins were rather higher in the Syntenin-1 KO mice. We also found that IgA and IgM production of splenic B cells stimulated in vitro was increased in Syntenin-1 KO mice. In addition, we showed that a distribution of intestinal microbial flora was influenced in Syntenin-1 KO mice. Our data indicate that Syntenin-1 negatively regulates the intestinal immunoglobulin production and has a function to maintain the intestinal homeostasis in vivo. The analysis of Syntenin-1 KO mice may provide novel information on not only mucosal immunity but also other functions of Syntenin-1 such as cancer metastasis and neural development.

• Authors: Kentaro Tamura, Masashi Ikutani, Taketoshi Yoshida, Ayumi Tanaka-Hayashi, Tsutomu Yanagibashi, Ran Inoue, Yoshinori Nagai, Yuichi Adachi, Toshio Miyawaki, Kiyoshi Takatsu, Hisashi Mori
• Journal: Immunobiology (Immunobiology) Vol. 220 Issue 5 Pg. 597-604 (May 2015) ISSN: 1878-3279 [Electronic] Netherlands
• PMID: 25543283 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 The Authors. Published by Elsevier GmbH.. All rights reserved.

Chemical References

• Immunoglobulins
• Interleukin-5
• Sdcbp protein, mouse
• Syntenins

Topics

• Animals
• Antibody Formation (genetics)
• Gastrointestinal Microbiome (genetics)
• Homeostasis (genetics)
• Immunity, Mucosal (genetics)
• Immunoglobulins (biosynthesis)
• Interleukin-5 (metabolism)
• Intestines (immunology, microbiology)
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Signal Transduction (genetics)
• Syntenins (genetics, metabolism)