Abstract | INTRODUCTION: METHODS: PS- liposomes (size, 100 nm or 200 nm) were PEGylated with PEG2000 or PEG5000 at 1 or 5 mol%, and radiolabeled with (111)In. For the study of uptake in vitro, liposomes were incubated with mouse peritoneal macrophages. Biodistribution studies in vivo were carried out in ddY mice. En face autoradiograms were obtained with apoE(-/-) mice upon intravenous injection of (111)In-liposomes. RESULTS: Uptake was decreased significantly at 5 mol% PEGylation in 100-nm PS- liposomes (*P<0.05 vs. 0 mol%). All the PEGylated liposomes tested showed significantly lower uptake than the non-PEGylated control in 200-nm liposomes. In vivo results showed slower blood clearance in PEGylated liposomes. Autoradiograms in apoE(-/-) mice were well matched with Oil Red O staining. Additionally, 200-nm PS- liposomes modified with 5% PEG2000 ([(111)In]5%PEG2000PS200) showed the highest uptake to the region in vivo. CONCLUSIONS: As expected, PEGylation retarded the rate of blood clearance. In addition, it affected liposome uptake by macrophages in vitro. These results suggest that the balance between the rate of blood clearance and macrophage recognition is important, and [(111)In]5%PEG2000PS200 showed the best results in our investigation.
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Authors | Mikako Ogawa, Ryuji Uchino, Ayumi Kawai, Mutsumi Kosugi, Yasuhiro Magata |
Journal | Nuclear medicine and biology
(Nucl Med Biol)
Vol. 42
Issue 3
Pg. 299-304
(Mar 2015)
ISSN: 1872-9614 [Electronic] United States |
PMID | 25533763
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier Inc. All rights reserved. |
Chemical References |
- Apolipoproteins E
- Indium Radioisotopes
- Liposomes
- Phosphatidylserines
- Polyethylene Glycols
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Topics |
- Animals
- Apolipoproteins E
(deficiency)
- Biological Transport
- Indium Radioisotopes
- Liposomes
(chemistry, metabolism, pharmacokinetics)
- Macrophages
(metabolism)
- Mice
- Phosphatidylserines
(chemistry)
- Plaque, Atherosclerotic
(diagnostic imaging)
- Polyethylene Glycols
(chemistry)
- Tissue Distribution
- Tomography, Emission-Computed, Single-Photon
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