miRNA-34a underexpressed in Merkel cell polyomavirus-negative Merkel cell carcinoma.

Abstract	Merkel cell polyomavirus (MCV) is frequently detectable in Merkel cell carcinoma (MCC) tumors, but the significance of MCV infection is not yet totally understood. Thus far, no key regulatory miRNA has been identified for MCC tumorigenesis. However, distinct miRNA expression profiles have been suggested for MCV-positive and MCV-negative tumors. We used microarray hybridization to identify miRNA expression differences in MCC tumor samples according to MCV status and further validated these results by quantitative reverse transcription polymerase chain reaction (qRT-PCR). When compared with MCV-negative tumors, we detected overexpression of miR-34a, miR-30a, miR-142-3p, and miR-1539 in those MCV positives. In addition, slight underexpression was detectable in MCV-positive tumors of miR-181d. We confirmed the distinct expression of miRNAs in MCV-positive and MCV-negative tumors and confirmed statistically significant underexpression of miR-34a in MCV-negative tumors by both array analysis and qRT-PCR. Neither tumor location nor development of metastases affected miRNA expression.
Authors	Tuukka Veija, Helka Sahi, Virve Koljonen, Tom Bohling, Sakari Knuutila, Neda Mosakhani
Journal	Virchows Archiv : an international journal of pathology (Virchows Arch) Vol. 466 Issue 3 Pg. 289-95 (Mar 2015) ISSN: 1432-2307 [Electronic] Germany
PMID	25491743 (Publication Type: Comparative Study, Journal Article)
Chemical References	Biomarkers, Tumor MIRN142 microRNA, human MIRN1539 microRNA, human MIRN30b microRNA, human MIRN34 microRNA, human MicroRNAs
Topics	Aged Aged, 80 and over Biomarkers, Tumor (genetics, metabolism) Carcinoma, Merkel Cell (metabolism, pathology) Down-Regulation Female Gene Expression Regulation, Neoplastic Humans Male Merkel cell polyomavirus MicroRNAs (genetics, metabolism) Microarray Analysis Middle Aged Reproducibility of Results Retrospective Studies Skin Neoplasms (metabolism, pathology)

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