Androgen receptors, androgen-dependent proliferation, and 5 alpha-reductase activity of small-cell lung cancer cell lines.

Abstract

We investigated the influence of testosterone on binding to established small-cell lung cancer (SCLC) cell lines and were able to show specific high-affinity and low-capacity binding sites in some cell lines with a typical receptor size, using sucrose density gradient centrifugation. In addition, we demonstrated marked growth stimulation with testosterone and dehydrotestosterone using different androgen-receptor-positive small-cell lung cancer cell lines. This growth stimulation could be counteracted by the addition of anti-androgens like cyproterone acetate or flutamide. The presence of 5 alpha-reductase activity, 17 beta-hydroxysteroid-dehydrogenase and 3 alpha-hydroxysteroid-dehydrogenase activities could be demonstrated, suggesting testosterone metabolism of small-cell lung cancer cell lines.

• Authors: M Maasberg, M Rotsch, G Jaques, U Enderle-Schmidt, R Weehle, K Havemann
• Journal: International journal of cancer (Int J Cancer) Vol. 43 Issue 4 Pg. 685-91 (Apr 15 1989) ISSN: 0020-7136 [Print] United States
• PMID: 2539332 (Publication Type: Journal Article)

Chemical References

• Androgen Antagonists
• Androgens
• Receptors, Androgen
• Tritium
• Oxidoreductases
• Cholestenone 5 alpha-Reductase
• Thymidine

Topics

• Androgen Antagonists (pharmacology)
• Androgens (metabolism, pharmacology)
• Carcinoma, Small Cell (metabolism, pathology)
• Cell Division (drug effects)
• Cell Line
• Centrifugation, Density Gradient
• Cholestenone 5 alpha-Reductase
• Dose-Response Relationship, Drug
• Humans
• Lung Neoplasms (metabolism, pathology)
• Oxidoreductases (analysis, metabolism)
• Radioligand Assay
• Receptors, Androgen (drug effects, metabolism)
• Thymidine (metabolism)
• Tritium
• Tumor Cells, Cultured