Acetylated derivative of glaucine inhibits joint inflammation in collagenase-induced arthritis.

Abstract

CONTEXT:

Osteoarthritis (OA) has become by far the most common joint disorder. A number of studies using OA animal models have explored the effects of agents that can modulate bone metabolism.

OBJECTIVE:

In the present study, we investigated the effect of acetylated derivative of plant alkaloid glaucine (ADG) on experimental OA in mice.

MATERIALS AND METHODS:

Arthritis was induced by two intraarticular (i.a.) injections of collaganase. Histopathological changes were observed through hematoxylin and eosine (H&E), safranin O and toluidine blue staining. Differentiation of bone marrow (BM) cells was evaluated by tartarate-resistant acid phosphatase (TRAP) assay. The expression of phospho-Janus kinase 2 (pJAK2) and phospho signal transducer and activator of transcription3 (pSTAT3) expression in the joints was determined by immunohistochemistry.

RESULTS:

We established that ADG significantly decreased cell infiltration (2.32 ± 0.14 versus 1.62 ± 0.13), cartilage loss (2.42 ± 0.12 versus 1.12 ± 0.10) and bone erosion (1.76 ± 0.13 versus 1.04 ± 0.14) in arthritic mice. It appeared that the substance inhibited in a dose-dependent manner osteoclast differentiation in vitro. ADG suppressed the expression of pJAK2 in the joint and partially affected the expression of pSTAT3.

CONCLUSION:

Present results suggest that ADG is a suitable candidate for further development as an anti-arthritic agent.

• Authors: Valeriya Gyurkovska, Stefan Philipov, Nadezhda Kostova, Nina Ivanovska
• Journal: Immunopharmacology and immunotoxicology (Immunopharmacol Immunotoxicol) Vol. 37 Issue 1 Pg. 56-62 (Feb 2015) ISSN: 1532-2513 [Electronic] England
• PMID: 25328086 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Aporphines
• Microbial Collagenase
• glaucine

Topics

• Acetylation
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, adverse effects, chemistry, therapeutic use)
• Aporphines (administration & dosage, adverse effects, chemistry, therapeutic use)
• Arthritis, Experimental (chemically induced, drug therapy, pathology)
• Bone Marrow Cells (drug effects)
• Cell Differentiation (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Joints (drug effects, enzymology, pathology)
• Male
• Mice, Inbred ICR
• Microbial Collagenase (pharmacology)
• Molecular Structure
• Osteoarthritis (chemically induced, drug therapy, pathology)
• Osteoclasts (drug effects, immunology, pathology)