Study on lead-induced activation of rat renal interstitial fibroblasts and the related mechanisms.

Abstract

CONTEXT:

Lead is a common industrial toxicant and has been proved to be associated with the kidney damage.

OBJECTIVE:

To investigate the effect and mechanism of lead on expression of rat renal interstitial fibroblast activation related protein.

MATERIALS AND METHODS:

The expression of activation related protein mRNA was measured by real-time PCR in the NRK/49F treated by lead acetate with different concentrations (0, 0.5, 1 and 2 µmol/L). The effects of lead acetate on the level of fibronectin (FN) and signal transduction factors (Smads protein) expression were observed by Western blot.

RESULTS:

The mRNA expression of activation-related protein increased significantly after the cells were stimulated by lead acetate for 24 h. The lead acetate-treated group could upregulate the p-Smad2, p-Smad3 and FN protein expression compared with the control group. The level of Smad2/3 protein expression did not change in all groups, the expression of SnoN decreased significantly compared with the control group.

DISCUSSION AND CONCLUSION:

Lead acetate could increase the mRNA expression of activation-related factors. It could promote inflammatory reaction induced by TGF-β via Smad signaling pathway. Lead acetate has the effect on inducing the renal fibrosis.

• Authors: Lin Han, Rong Zhou, Xingya Kuang, Xuefang Feng, Mindong Chen, Jie Shen
• Journal: Toxicology mechanisms and methods (Toxicol Mech Methods) Vol. 24 Issue 9 Pg. 713-8 (Dec 2014) ISSN: 1537-6524 [Electronic] England
• PMID: 25270974 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers
• Lead

Topics

• Animals
• Base Sequence
• DNA Primers
• Fibroblasts (drug effects, metabolism)
• Kidney (cytology, drug effects, metabolism)
• Lead (toxicity)
• Polymerase Chain Reaction
• Rats