[Effects of soothing liver and invigorating spleen recipes on SREBP-1c, SCD-1 mRNA and proteins expression in hepatocytes of NAFLD rats].

Abstract

OBJECTIVE:

To observe the effects of soothing liver and invigorating spleen recipes on the expression levels of SREBP-1c, SCD-1 mRNA and proteins in hepatocytes of NAFLD rats,and to explore its possible mechanisms of prevention and treatment of NAFLD.

METHODS:

75 SD male rats were randomly divided into 5 groups: normal group, model group, oothing liver group (administrated with 9.6 g/kg), invigorating spleen group (administrated with 30 g/kg)and integrated group (administrated with 39.6 g/kg). The rats of NASH model were induced by feeding a high-fat diet. After treatment for 8 weeks,9 rats were randomly taken to detect liver function, TC, TG and pathological changes in liver tissue. The other 6 rats of each group were taken respectively and collagenase (Type IV) was perfused to digest liver tissue with the circulation in vitro to separate hepatocytes. Real-time Q-PCR and Western Blot were used to detect the expression levels of SREBP-1c, SCD-1 mRNA and proteins.

RESULTS:

Compared with the model group,the different decrease levels of SREBP-1c, SCD-1 genes and proteins were found in all drug therapy groups (P < 0.05 or P < 0.01), as well different degrees that liver lipid and pathological changes became better, especially that of in soothing liver group. Comparison between the all drug therapy groups,the hepatocytes expression levels of SREBP-1c and SCD-1 mRNA in soothing liver group were lower than that of in invigorating spleen group (P < 0.05), but expression levels of the proteins had no statistical significances.

CONCLUSION:

Soothing liver and invigorating spleen recipes prevent and treat NAFLD,its mechanism may be related to inhibiting the activation of SREBP-1c/SCD-1 signal pathway in hepatocytes to down-regulate TC and TG synthesis and reduce hepatic lipid deposition. SREBP-1c, SCD-1 mRNA and proteins may be the effective targets.

• Authors: Yong-jian Xu, Qin-he Yang, Li Han, Yu-pei Zhang, Yi-zhen Liu, Ling Jin, Hai-zhen Yan
• Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials (Zhong Yao Cai) Vol. 37 Issue 1 Pg. 80-6 (Jan 2014) ISSN: 1001-4454 [Print] China
• PMID: 25090711 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drug Combinations
• Drugs, Chinese Herbal
• RNA, Messenger
• Srebf1 protein, rat
• Sterol Regulatory Element Binding Protein 1
• Stearoyl-CoA Desaturase

Topics

• Animals
• Diet, High-Fat (adverse effects)
• Disease Models, Animal
• Down-Regulation
• Drug Combinations
• Drugs, Chinese Herbal (pharmacology)
• Hepatocytes (drug effects, metabolism, pathology)
• Liver (drug effects, metabolism, pathology)
• Liver Function Tests
• Male
• Non-alcoholic Fatty Liver Disease (metabolism, pathology)
• Plants, Medicinal (chemistry)
• RNA, Messenger (genetics, metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Stearoyl-CoA Desaturase (genetics, metabolism)
• Sterol Regulatory Element Binding Protein 1 (genetics, metabolism)