MiR-9 promotes microglial activation by targeting MCPIP1.

Abstract

Microglia participate in innate inflammatory responses within the central nervous system. The highly conserved microRNA-9 (miR-9) plays critical roles in neurogenesis as well as axonal extension. Its role in microglial inflammatory responses, however, remains poorly understood. Here we identify a unique role of miR-9 in mediating the microglial inflammatory response via distinct signalling pathways. MiR-9-mediated regulation of cellular activation involved downregulated expression of the target protein, monocyte chemotactic protein-induced protein 1 (MCPIP1) that is crucial for controlling inflammation. Results indicate that miR-9-mediated cellular activation involved signalling via the NF-κB pathway, but not the β-catenin pathway.

• Authors: Honghong Yao, Rong Ma, Lu Yang, Guoku Hu, Xufeng Chen, Ming Duan, Yeonhee Kook, Fang Niu, Ke Liao, Minggui Fu, Gang Hu, Pappachan Kolattukudy, Shilpa Buch
• Journal: Nature communications (Nat Commun) Vol. 5 Pg. 4386 (Jul 14 2014) ISSN: 2041-1723 [Electronic] England
• PMID: 25019481 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• MIRN9 microRNA, mouse
• MIRN9 microRNA, rat
• MicroRNAs
• NF-kappa B
• beta Catenin
• Ribonucleases
• Zc3h12a protein, mouse

Topics

• Animals
• Blotting, Northern
• Blotting, Western
• Flow Cytometry
• In Situ Hybridization
• Male
• Mice
• Mice, Inbred C57BL
• MicroRNAs (genetics, metabolism)
• Microglia (metabolism)
• NF-kappa B (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley
• Real-Time Polymerase Chain Reaction
• Ribonucleases (genetics, metabolism)
• Signal Transduction (physiology)
• beta Catenin (genetics, metabolism)