Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population.
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| Abstract |
Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved in these effects.
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| Authors | Lauren J Wegman-Points, Melissa L T Teoh-Fitzgerald, Gaowei Mao, Yueming Zhu, Melissa A Fath, Douglas R Spitz, Frederick E Domann |
| Journal | Redox biology (Redox Biol) Vol. 2 Pg. 847-54 ( 2014) ISSN: 2213-2317 [Print] Netherlands |
| PMID | 25009786 (Publication Type: Journal Article, Research Support, N.I.H., Extramural) |
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